We present techniques for the preparation of metaphase sea urchin chromosomal karyotypes and preliminary karyograms
for both 5.
A limited number of these plates were used to plot karyograms
as this technique, which is very accurate but long, was completed by flow cytometry DNA assay.
showing t(9;22)(q34;q11.2) with concurrent trisomy 19.
obtained by arranging the chromosomes in order of decreasing size and centromere position is shown on Figure 1c.
However the t(8;8) fusion can be cryptic and may not be seen in all the karyograms
. According to the current World Health Organization classification of soft tissue tumors, the consistent molecular detection of the HEY1-NCOA2 fusion in EMCS establishes it as a marker of potential clinical utility.
All the liver cancer tissues showed abnormal karyograms
with fat vacuoles.
For each proband, 30 metaphases were counted and 5 metaphases were analyzed and karyograms
were prepared using the Cytovision computer-assisted karyotyping system version 4.1 (Applied Imaging, NewCastle Upon Tyne, UK).