In SLNB techniques various studies like Giuliano et al used Isosulfan blue
dye (4 ml of 1% sigma--Aldrich Milwaukee W1 USA)
Many techniques have been used for lymphatic mapping, such as blue dye (Isosulfan blue
, PBD V, or methylene blue), radiocolloid, or both in combination.
Localization of Nonpalpable Breast Lesions Using Hook-Wire Combined with Isosulfan Blue
In order to localize SLN, either a combination of direct visualization technique, employing Isosulfan Blue
dye and scintigraphy, is used4-6 or scintigraphic technique alone is employed7.
The stains used most frequently for detection of sentinel lymph nodes include isosulfan blue
, patent blue, and methylene blue.
If dual tracer is desired by the surgeon, isosulfan blue
or diluted methylene blue dye is also injected either peritumorally or into the subareolar plexus.
Visual identification of blue dye, most frequently isosulfan blue
, is the "colorimetric method" and has been used most commonly with cervical injection for endometrial cancer.
Aurobindo, which secured approvals for isosulfan blue
single-dose vials and tranexamic acid injection from FDA this year, is working on penem products, non-SSP type antibiotics and anti-anxiety drugs.
Combined use of radioactive colloid and blue dye injection is considered as gold standard for axillary sentinel lymph node biopsy (SLNB) in breast cancer with 97% accuracy rate,8-10 but this combine usage does not attain an adequately higher detection rate to defend the cost.11 While some researchers have been using blue dye only for identification of SLN with good reliability.12 The positive results found by using methylene blue dye and by isosulfan blue
dye were 99% and 97% respectively.13,14 While another similar study for methylene blue dye was done showing the sensitivity and specificity of 85.7% and 71.4% respectively.15
Moreover they used isosulfan blue
to ensure correct and definitive ligation of lymphatic vessels.
Sentinel lymph nodes were identified by isosulfan blue