The isosbestic point 230 nm was found to be satisfactory (Fig.
The validated LC method was found to be accurate and applicable for the simultaneous determination of citalopram (Pramcit 20 mg) with piroxicam (Feldene 10 mg), celecoxib (Celbex 100 mg) and diclofenac sodium (Dicloran 50 mg) in commercial formulations showing good separation with high resolution for all the analytes at the isosbestic point.
Elucidation of a photoproduct was shown through an isosbestic point
on UV-vis and through the absorhance of a photoproduct peak on the HPLC plots.
The maximum absorbance is affected by temperature; however, temperature dependency can be avoided by making the absorbance measurements at a temperature isosbestic point
(near 1000 nm).
This difference is minimal at 610 nm, an isosbestic point
Furthermore, spectroscopic titration studies for the interaction of these food additives with DNA showed that these dyes bind to calf thymus DNA and distinct isosbestic points
are observed clearly suggesting binding of the dyes to DNA.
The retina is illuminated through the pupil, preferably at isosbestic points
for oxy- and deoxyhemoglobin, using several discrete visible/NIR light bands while the reflected light from vessels overlying the optic disk is analyzed.
One chooses a combination of absorption maxima and isosbestic points
, with a total number of wavelengths equal to the number of components of interest.