isosbestic point


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i·sos·best·ic point

in applied spectroscopy, a wavelength at which absorbance of two substances, one of which can be converted into the other, is the same.

i·sos·best·ic point

(ī'sos-bes'tik poynt)
applied spectroscopy A wavelength at which absorbance of two substances, one of which can be converted into the other, is the same.
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References in periodicals archive ?
Fura-2 fluorescence was alternately excited at the isosbestic point (357 nm) and at the calcium-sensitive wavelength (.
Overlapping the absorbance spectra for mixtures of ITX and MA having differing irradiation times between 0 and 60 min showed that a single isosbestic point was produced at approximately 350 nm (Fig.
6), we scanned the excitation spectra of the coproporphyrin calibrator and the real fecal samples from 340 to 450 nm, while monitoring the emission at 668 nm, the emission isosbestic point for coproporphyrin and protoporphyrin.
The maximum absorbance is affected by temperature; however, temperature dependency can be avoided by making the absorbance measurements at a temperature isosbestic point (near 1000 nm).