The isosbestic point 230 nm was found to be satisfactory (Fig.
The validated LC method was found to be accurate and applicable for the simultaneous determination of citalopram (Pramcit 20 mg) with piroxicam (Feldene 10 mg), celecoxib (Celbex 100 mg) and diclofenac sodium (Dicloran 50 mg) in commercial formulations showing good separation with high resolution for all the analytes at the isosbestic point.
All the three redox pairs have isosbestic
points at 230-240 nm suggesting a minimal interference with the robust signal of conjugated dienes.
Elucidation of a photoproduct was shown through an isosbestic
point on UV-vis and through the absorhance of a photoproduct peak on the HPLC plots.
The retina is illuminated through the pupil, preferably at isosbestic
points for oxy- and deoxyhemoglobin, using several discrete visible/NIR light bands while the reflected light from vessels overlying the optic disk is analyzed.
point 158 may, presumably, be due to equal increase of DNA fragments by the probable DNA damage with the increasing in concentration of the compound- DNA complex.
6), we scanned the excitation spectra of the coproporphyrin calibrator and the real fecal samples from 340 to 450 nm, while monitoring the emission at 668 nm, the emission isosbestic
point for coproporphyrin and protoporphyrin.
The maximum absorbance is affected by temperature; however, temperature dependency can be avoided by making the absorbance measurements at a temperature isosbestic
point (near 1000 nm).
This difference is minimal at 610 nm, an isosbestic
One chooses a combination of absorption maxima and isosbestic
points, with a total number of wavelengths equal to the number of components of interest.