isopropylthiogalactoside

i·so·pro·pyl·thi·o·ga·lac·to·side (iPrSGal, IPTG),

(ī'sō-pro'pil-thī'ō-gă-lak'tō-sīd),
An artificial galactoside capable of inducing β-galactosidase in Escherichia coli without being split, as are the natural substrates such as lactose.
Farlex Partner Medical Dictionary © Farlex 2012
References in periodicals archive ?
When the optic density reached a value of 0.6, the culture was induced with isopropylthiogalactoside 0.1 M (Gibco BRL, Gaithersburg, MD, UsA) and incubated at 30[degrees] C under agitation for 4 h.
coli BL21 (DE3) GOLD carrying pET28a-SCO1678 was grown in LB supplemented with tetracycline and kanamycin until OD600 reached 0.5; then the culture was induced with 1mM IPTG (isopropylthiogalactoside) and incubated for six hours at 22[degrees]C.
The overnight culture was transferred to 225 mL 2 x YT broth and grown with shaking to midlog phase ([A.sub.600] 0.6) before inducing TbHsp70.c protein expression by the addition of isopropylthiogalactoside (IPTG) to a final concentration of 1 mM.