Ehrhardt, "Improved staining of proteins in polyacrylamide gels including isoelectric focusing
gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250," Electrophoresis, vol.
The protein fractions showing considerable variations were subjected to isoelectric focusing
. High molecular weight protein fraction of 270 kDa was resolved into two bands corresponding to isoelectric points 5.9 and 6.1(Fig.
of human hemoglobin: its application to screening, to the characterization of 70 variants, and to the study of modified fractions of normal hemoglobins.
Protein mapping by combined isoelectric focusing
and electrophoresis of mouse tissues.
We fractionated cells by preparative isoelectric focusing
in a pH gradient of 3-10 and bioassayed the resultant fractions in test diets to determine their effects on egg produc tion.
The new system, which fully automates all processes from isoelectric focusing
to polyacrylamide gel electrophoresis to detection, can perform two-dimensional electrophoresis of proteins in about one hour.
This protein was found to be present in numerous cell cultures so I purified to homogeneity by preparative isoelectric focusing
and SDS polyacrylamide gel electrophoresis this protein from human embryonic kidney cells (i.e., HEK293).
was performed in vertical slab gels.
The technique gives simultaneous focusing and separation of differently charged analytes in a manner analogous to isoelectric focusing
of proteins, but is much simpler to implement than isoelectric focusing
and has the additional advantage of working with any charged analyte, rather than just proteins.
(IEF) in agarose gels revealed species-specific protein profiles in the ten control species.
High resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing
. Plant Physiology 60:787-93.
Available as an upgrade module, the detector will also be integrated into four application-specific CE systems designed for automated and quantitative isoelectric focusing
, oligosaccharide and monosaccharide analysis, DNA-protein interaction analysis, gene expression, and viral load determination.