The enzyme catalysed acetic anhydride hydrolysis, and subsequent esterification with isoamyl
alcohol was conducted in a microreaction platform.
To a solution of 2-(o-aminophenyl)oxazoline (3) (1 equiv.) in methanol, a solution of isoamyl
nitrite (8 equiv.) in methanol was added.
Hexyl acetate increased with crop reduction in 2011 but was reduced in 2012, and the same occurred for isoamyl
acetate and terpinolene.
acetate production catalyzed by H3PW12O40 on their partially substituted Cs or K salts.
7) The above supernatant containing DNA was treated with equal volume of isoamyl
alcohol at -20degC for 12 h
After the lysis step, DNA was purified with phenol: chloroform: isoamyl
alcohol (25:24:1) and in later the aqueous phase was treated twice time with phenol: chloroform (25:24) to purify the genomic DNA (Table 1).
Photo allergens After radiation Tetrachloro salicylanilide + Chlorhexidine + Triclosan + Methylcoumarin + Musk ambrette + Thiourea + Isoamyl
p-methoxycinnamate + Ethylhexyl p-methoxycinnamate + Benzophenone 4 + Oxybenzone + 4-tert-butyl-4'-methoxy-dibenzoylmethane + Benzydamine + Sulfanilamide + Chlorpromazine + Promethazine + Hydrochlorothiazide + Carbamazepine + Piroxicam + Lichen acid mix + P-aminobenzoic acid + + = positive irradiated photo-epicutaneous test Discussion
Cachaca samples (at 42[degrees]GL) were analysed for copper, with direct measuring by atomic absorption spectrometry--Perkin-Elmer 373 (AOAC, 1995); acetaldehyde, ethyl acetate, methanol, n-propanol, isobutanol and isoamyl
alcohol, using gas chromatography with a flame ionization detector (INSTITUTO ADOLFO LUTZ, 1985); pH, with direct measuring in a digital pH-meter; alcohol content ([degrees]GL), using a digital densimeter; total, fixed and volatile acidity, by titration (AMORIM et al., 1979).
Add equal volume of isoamyl
alcohol to the supernatant and centrifuge at 12000 rpm for 10 minutes.
Extraction buffer: 120 mM Tris-HCl, pH 8.0; 80 mM EDTA pH 8.0; 4% [beta]-mercaptoethanol; 2% CTAB Clean up buffer: 120 mM Tris-HCl pH 8.0; 10 mM EDTA pH 8.0; 2% [beta]-mercaptoethanol; 2% PVP; 1.5 M NaCl; 0.2% CTAB 5M Potassium acetate pH 4.8 3M Sodium acetate pH 5.2 70% (v/v) ethanol Chloroform- isoamyl
alcohol (24: 1 v/v) 10 mg/ml RNAase TE: 10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0 DNA extraction procedure
The tube was incubated for 2 h at 56[degrees]C before adding 200 [micro]l phenol-chloroform: isoamyl
alcohol (25:24:1 by volume).