ion-exchange chromatography


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ion-ex·change chro·ma·tog·ra·phy

(ī'on-eks-chānj' krō'mă-tog'ră-fē)
Chemical investigation in which cations or anions in the mobile phase are separated by electrostatic interactions with the stationary phase.
See also: anion exchange, cation exchange
References in periodicals archive ?
Xing et al., "High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography," Journal of Separation Science, vol.
filiformis was dissolved in 0.05 M sodium acetate buffer (pH 5.0) and then fractionated by ion-exchange chromatography on DEAE-cellulose column (1.0 x 24.5 cm) previously equilibrated and washed with the same buffer, followed by separation of fractions of [iota]-CARs also using the same buffer containing NaCl at different concentrations (0.5, 0.75 and 1 M).
The partially purified of enzyme sample was then subjected to ion-exchange chromatography through Q-Scpharose.
There have been many studies involving speciation by the coupling of ion-exchange chromatography (IC) to ICP-MS (IC-ICP-MS) (6,7); however, studies reported in the literature have employed two separate columns (anionic and cationic).
AAA's are based on ion-exchange chromatography and are used for a variety of applications, such as the analysis of complex hydrosylates, quality control in drug synthesis, and research and metabolic disorder screening.
In technology terms, ion-exchange chromatography had the highest consumption in past few years.
The extracted Protease and Xylanase were subjected to purification first by Dialysis followed by Ion-exchange chromatography. The Protease and Xylanase enzymes from isolated fungus were dialyzed by the method of Scope (1994).
The size exclusion and ion-exchange chromatography results further revealed two specific major peaks corresponding to the two hemocyanin subunits.
A monomeric 5.5-kDa protein with hemolytic activity toward rabbit erythrocytes was isolated from seeds of Albizia lebbeck by using a protocol that involved ion-exchange chromatography on Q-Sepharose and SP-Sepharose, hydrophobic interaction chromatography on Phenyl-Sepharose, and gel filtration on Superdex 75.
TSPs (33 mg) were first dissolved in 50 mM AcNa buffer (2 mg [mL.sup.-1]) and subjected to ion-exchange chromatography in a DEAE-cellulose column (Sigma Chemical) equilibrated and percolated with 50 mM AcNa buffer until complete removal of non-retained polysaccharides, followed by SP fractioning by elution with the same equilibrium buffer containing NaCl in different concentrations (0.50, 0.75 and 1.00 M) using a fraction collector (FRAC-920) with flow adjusted to 60 mL [h.sup.-1].