iodoacetamide


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i·o·do·a·cet·a·mide

(ī-ō'dō-ă-sē'tă-mīd),
ICH2-CONH2; a cerivative of acetamide that reacts readily with sulfhydryl groups and is therefore a strong inhibitor of many enzymes.
References in periodicals archive ?
In support of the idea that TRPA1 is activated by electrophiles through modification of these three Cys residues, a mass spectrometric analysis revealed that the irreversible alkylating agent iodoacetamide modifies Cys415, Cys422 and Cys622 localized in the N-terminal ARD of mouse TRPA1.
WORDS WITH 2 EACH OF 4 DIFFERENT VOWELS iooaeaie IODOACETAMIDE uaioouia QUASICOLLOQULAL ueuieaia UNSESQUIPEDALIAN ueouioie UNREVOLUTIONIZED auaeoeou AQUAEOLEOUS
Each sample (between 30 [micro]g and 200 [micro]g) was first reduced with dithiothreitol and alkylated with iodoacetamide and then digested by adding 2 [micro]g sequencing-grade modified trypsin overnight at 37[degrees]C.
8, 2% (m/v) SDS, 20% (v/v) glycerol, 270 mM iodoacetamide (IAA)], and Anally rinsed with water.
Tenders are invited for Supply Of Consumables For Proteomics, Chaps - Qty : 1 Pack X 10 Mg, Iodoacetamide - Qty : 1 Pack X 25 Gm
Ammonia bicarbonate (ABC), ammonium persulfate (AP), acetronitrile (ACN), 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate (CHAPS),DNBS, formic acid (FA), iodoacetamide (IAM), and cell lysis solution were from Sigma (US).
5 M iodoacetamide was added, and proteins were alkylated at room temperature for 30 min, in the dark.
4 (final concentration of iodoacetamide was 17 mM), and incubated at 37 oC for half an hour in the dark.
Iodoacetamide (IAA) and phosphatase inhibitor cocktail were purchased from Sigma (St.
8, 6 M urea, 30% glycerol, 2% SDS, 1% DTT) and subsequently alkylated for 20 min in equilibration buffer containing 5% iodoacetamide instead of DTT at room temperature.
Samples were reduced with dithiothreitol (30 min at 37[degrees]C), and cysteine sulfhydryls were alkylated with iodoacetamide (30 min at 37[degrees]C in darkness).