two-photon imaging: a versatile tool for dissecting the immune system," Annals of the Rheumatic Diseases, vol.
microscopy demonstrated that TNFa-induced leukocyte adhesion and emigration was blunted in EC-specific Panx1-deficient mice. In a similar study, thrombin induced a robust, Ca 2+-dependent ATP release through Panx1 channels in HUVECs. These results highlight Panx1 as a key protein for the cross talk between cytokine and purinergic signaling in early inflammation.
In this regard, one of the promising methods is acoustic microscopy, which has several advantages: being noninvasive, intravital
, non-toxic, and allows observation in dynamics.
two-photon microscopic analysis showed that T cell migration in the parenchyma is significantly attenuated in the conditional ATX-deficient mice compared to control mice and that the ATX/LPA-dependent T cell motility is mediated by the LPA receptor [LPA.sub.2] on the T cell surface.
Researchers made the discovery through the use of a technology called two-photon intravital
According to the results of intravital
angiographic study, the grafts were passable and conducted a pulse wave without difficulty; the prosthesis boundaries were barely distinguishable; there were no signs of stenosis or dilatation (Figure 6).
Westermann, "Defining the quantitative limits of intravital
two-photon lymphocyte tracking," Proceedings of the National Academy of Sciences of the United States of America, vol.
Mesenchymal stem cells and intravital
microscopy," Microvascular Research, vol.
microscopic methods to evaluate anti-inflammatory effects and signaling mechanisms evoked by hydrogen sulfide," in Methods in Enzymology, vol.
The imaging site was determined using an intravital
microscope, and a catheter was inserted into a vessel upstream of the set site to obtain a perfusion route.
imaging of hair-cell development and regeneration in the zebrafish," Frontiers in Neuroanatomy, vol.