Litters of 2-day-old Swiss Albino suckling mice (8 mice/ litter) were intracerebrally
inoculated using the indicated virus titers or cell culture media as a control.
In order to develop mouse brain adapted stock, 1 to 4 days old suckling Swiss Albino mice (6 mice per group) were inoculated intracerebrally
with 20 [micro]l of respective DENV suspension and monitored for 21 days for the appearance of signs such as paralysis or difficulty in walking.
The first group was inoculated intramuscularly with a viral dilution of 10 (9.1) (LD50), and the second group was inoculated intracerebrally
with a dilution of 10 (4.1) (LD50).
Neuro 2a, one of the several clonal derivatives of the C-1300 spontaneous neuroblastoma of A/J mice, has been transplanted in various sites, including intracerebrally
, for evaluating multiple therapeutic modalities.
From left to right the 1st, 2nd, 3rd, and 4th columns correspond, respectively, to subjects intracerebrally
(i.c.) injected with normal brain homogenate, NBH, with NBH and nasal instilled with Piry virus suspension, NBH+Py, i.c., injected with ME7 infected brain homogenate, ME7, i.c., injected with ME7 and nasal instilled with Piry virus suspension, ME7+Py.
(22) Unknown 46 tumor-bearing mice settings following inoculated intracerebrally
with MDA-MB-231-BR- luciferase- expressing cells Bosetti et al.
The dilutions were injected intracerebrally
to the chick.
The neonatal mice were intracerebrally
inoculated with 20 [micro]L of 10-fold serially diluted virus or mock inoculated with DMEM.
(1996)  examined the satiety effect of GLP-1 on the central CNS in fasted rats injected intracerebrally
, in the ventricular area, with GLP-1 versus saline (control group), and measured food consumption at regular intervals.
Walker, "Pathogenesis of scrapie (strain 263k) in hamsters infected intracerebrally
, intraperitoneally or intraocularly," Journal of General Virology, vol.
Furthermore, in an aggressive prolactinoma generated in the multiple endocrine neoplasia 1 mouse model, Mab G6-31, a monoclonal anti-VEGF antibody, inhibited the growth of the intracerebrally
injected pituitary adenoma and reduced prolactin levels .
(ii) Insect bioassay: Toxorhynchites splendens mosquito larvae were inoculated intracerebrally
, incubated for 7-10 days at 29[degrees]C and then tested by the indirect immuno-fluorescence assay (IFA) on head squeeze preparations (Toxo-IFA).