The population of Puma concolor in south Florida is still so small that inbreeding will likely become common again among the intercrossed
descendants within the next few generations.
A total of 149 plants from the top 26 families were intercrossed
to produce Cycle 4 (FLMR4).
In the control lines for intraspecific and interspecific competition (BC and RC, respectively), 10 of the 100 plants were chosen randomly each generation and randomly intercrossed
to produce seed for the next generation.
Approximately 40 of the most vigorous and agronomically desirable plants (10 from each replication) within each cultivar were removed from the field in April and intercrossed
. An equal amount of seed from each plant was bulked to form the four Cycle 1 populations.
or females are crossed to wild-type males; [F.sub.1] females and males are intercrossed
; [F.sub.2] or females are backcrossed to wild-type males and the cycle repeated.
[F.sub.1]B[C.sub.5] plants with root-knot resistance were intercrossed
. Nematode resistant [F.sub.2]B[C.sub.5] plants were individually test crossed to a susceptible line.
In further crosses made in 1999, intercrossed
heterozygous normal plants produced progeny segregating a close fit to a 3:1 ratio (124:42).
Beginning in 2000, 12 independently derived [T.sub.0] plants were intercrossed
by hand in a polycross (Fehr, 1987), and 323 [T.sub.1] seed harvested.
Plants are selected with similar characteristics, intercrossed
, and their progeny are tested in turf plot evaluations.
Plants selected from Citation II, Citation III, and breeding populations used in their development were intercrossed
and subjected to additional cycles of population improvement in both Oregon and New Jersey.
In general, 20 selected families were intercrossed
to form a new cycle.
Thirteen inbred lines of approximately AES100 to AES300 maturity were intercrossed
to develop NDSM (Cross and Wanner, 1991).