standardization: leading to measures of insulin sensitivity and secretion for practical clinical care.
The intra- and the interassay coefficients of variation for the insulin assay
However, HOMA was not so accurate, as it was highly affected by the technical limitations of conventional insulin assays
: the cross-reactivity with proinsulin and lack of acceptable sensitivity to measure low fasting insulin levels; hence, its performance depends on insulin assay
and could be improved by using highly sensitive and specific method [8-10].
: The insulin assay
was done using the DSL-10-1600 ACTIVE Insulin ELISA kit (enzyme linked immunosorbent assay) which is an enzymatically "one step" sandwich-type immunoassay.
There are variations in the cutoff values of HOMA for insulin resistance due to the lack of established standards for population based studies as well as variability of insulin assay
The enzyme-linked immunosorbent assay (ELISA) kit for insulin assay
was purchased from Mercodia (Uppsala, Sweden).
The intra-assay and the inter-assay coefficient of variation for insulin assay
An average insulin assay
binding of 30% was achieved using an initial 1:5,000 dilution of insulin.
We determined the levels of plasma insulin by enzyme-linked immunosorbent assay (ELISA) using a mouse insulin assay
kit from Mercodia AB (Uppsala, Sweden).
Thomas, Department of Biochemistry, Bristol Royal Infirmary, Bristol, for insulin assay
and Dr J.
Serum insulin assay
showed that BCL enhanced the secretion of insulin significantly (Fig.
Reproducibility of insulin assay
was determined in two samples.