His GAD-65 and insulin antibody
levels were normal at <5 U/mL.
Although lipohypertrophy is not believed to be caused by immunological factors, insulin antibody
titres have been reported to correlate with the degree of lipoatrophy and lipohypertrophy in young people with type 1 diabetes .
The insulin was measured by RIA using human insulin radiolabelled with [sup.125]I as tracer, rat insulin as standard (Crystal Chem Inc., USA), and rat insulin antibody
(donated by Dr.
(sc-9168) was from Santa Cruz Biotechnology (Dallas, TX).
Prediluted primary anti-mouse insulin antibody
(1:1000), anti-mouse glucagon antibody (1:2000) (Sigma, USA).
Another study published in 2007 reported a similar insulin antibody
response--and placental passage--in women with gestational diabetes mellitus (GDM) who were receiving human insulin (Diabetes Care 1997;20:1172-5).
Once insulin is exogenously administered (for >10 days) the IAA determination is no longer valid because exogenous insulin injection can elicit insulin antibody
responses that cannot be distinguished from autoantibody production.
levels and other laboratory tests monitoring safety were similar for both groups.
Observations were made regarding insulin antibody
levels, weight gain, body mass index, and hypoglycemic events.
The striking facts in terms of the present cases were the presence of negative ICA and anti-GAD antibodies in both cases and the increased insulin antibody
titers at the end of the 3rd year in the 74-year-old female during the time period that the hypoglycemic attacks occurred.
Results of insulin antibody
monitoring during phase II and phase III clinical studies of inhaled insulin (Exubera) in patients with type 1 or type 2 diabetes (abstract 46).