insertional inactivation

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in·ser·tion·al in·ac·ti·va·tion

a technique of recombinant DNA technology used to select bacteria that carry recombinant plasmids; a fragment of foreign DNA is inserted into a restriction site within a gene for antibiotic resistance, thus causing that gene to become nonfunctional.

Farlex Partner Medical Dictionary © Farlex 2012

insertional inactivation

insertion of a piece of DNA into the coding sequence of a GENE, which is thereby inactivated. It is often used to identify recombinant VECTORS (2) in GENE CLONING and in turn to distinguish a recombinant vector from a non-recombinant vector. For example, insertion of a piece of foreign DNA into a cloning site which is located on an antibiotic-resistant gene on the vector can lead to loss of the antibiotic resistance PHENOTYPE by insertional inactivation. The recombinant vector will therefore specify antibiotic sensitivity, whilst the non-recombinant vector will specify antibiotic resistance.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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IPTG

References in periodicals archive ?

In vivo emergence of colistin resistance in Klebsiella pneumoniae producing KPC-type carbapenemases mediated by insertional inactivation of the PhoQ/PhoP mgrB regulator.