* A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-d 1 .
* A novel A-isoform-like inositol 1,4,5-trisphosphate 3-kinase from chicken erythrocytes exhibits alternative splicing and conservation of intron positions between vertebrates and invertebrates .
Bcl-2 functionally interacts with inositol 1,4,5-trisphosphate
receptors to regulate calcium release from the ER in response to inositol 1,4,5-trisphosphate
We began elucidating the mechanism by demonstrating that the same concentration of CE alters endothelial monolayer resistance, intracellular calcium ion flux, and the generation of inositol 1,4,5-trisphosphate
(data not shown).
They especially focused on the responses of inositol 1,4,5-trisphosphate
and ryanodine receptors (I[P.sub.3]R and RyR, respectively), which are known thimerosal targets.
They mediate the ER-associated degradation (ERAD) pathway which is responsible for the degradation of misfolded proteins in the ER such as activated inositol 1,4,5-trisphosphate
(IP[sub]3) receptors and other substrates in mammalian cells.
Expression of early growth response 1 (EGR1), MMP1, synaptotagmin 7 (SYT7), inositol 1,4,5-trisphosphate
3kinase a (ITPKA), CCL2, neurotrimin (NTM), insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), neuropilin 1 (NRP1), potassium channel tetramerization domain-containing protein 12 (KCTD12), heat-shock 27 kD protein 3 (HSPB3), transmembrane 4 L six family member 20 (TM4SF20), regulator of G protein signaling 4 (RGS4), NAMPT, COX2, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected by real-time PCR using the iCycler iQ detection system (Bio-Rad Laboratories), SYBR Green (Bio-Rad Laboratories), and specific primers (QuantiTect Primer Assay, Qiagen).
Transcriptional and immunocytochemical analyses show that murine myeloid immature DCs (IDCs) and mature DCs (MDCs) express inositol 1,4,5-trisphosphate receptor (I[P.sub.3]R) and ryanodine receptor (RyR) [Ca.sup.2+] channels, known targets of THI.
THI contains an oxidized mercury atom ([Hg.sup.2+]) whose redox properties can enhance the activity of the inositol 1,4,5-trisphosphate receptor (I[P.sub.3]R) and ryanodine receptor (RyRs), both intracellular [Ca.sup.2+] channels (Kaplin et al.
[Ca.sup.2+] levels in the ER are regulated by [Ca.sup.2+]-ATPase pumps, inositol 1,4,5-trisphosphate
(IP3) receptors, ryanodine receptors, and [Ca.sup.2+]-binding proteins (Orrenius et al.