inoculation test

inoculation test

An obsolete test in which monkeys were injected with the cerebrospinal fluid of patients with soccidianomyleitis, the results of which were regarded as positive if meningitic or paralysing signs appeared within a week.
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Clear behavioral changes along with paralysis of tail and hind legs were noticed in the mice of both groups between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT).
When the plants went into dormancy and during the assessment in the following year, no symptoms of the disease on the twigs had been detected for the first inoculation test.
The mouse inoculation test was conducted by using brains of 13 bats that died during capture and transport (9).
Three replicate samples of each inoculation test (24 h before & after) were obtained.
USDK-CBB-15, in a greenhouse leaf inoculation test conducted at Kimberly, ID, in January 2004, had a mean disease score of 2 on the basis of a 1-to-9 scale where I is no visible infection and 9 is completely susceptible.
Historically, the rabbit inoculation test is the gold standard for detecting very low titers of Treponema pallidum.
The clear supernatant of each sample was maintained at 4[degrees]C and then inoculated in a dose volume of 0.03 ml by intracerebral route, according to the standard mouse inoculation test (Koprowski, 1996).
Complete autopsy was not authorized by the family, but a postmortem retro-orbital brain sample confirmed rabies diagnosis by FAT, isolation on neuroblastoma cells, RREID, and mouse inoculation test. Mouse inoculation tests with saliva and CSF remained negative.
In order to confirm the presence of viruses in the inoculated plants, back inoculation test was conducted on 5 inoculated test plants of each cultivar by single plant inoculation method (Mishra et al., 1976).
The mouse inoculation test showed typical rabies sign as compared to control mice which did not show any such signs.