Energy metabolism of the inner cell mass
and trophectoderm of the mouse blastocyst.
Bongso, "Human feeders support prolonged undifferentiated growth of human inner cell masses
and embryonic stem cells," Nature Biotechnology, vol.
The procedure of Thouas et al11 was carried out for the differential staining of inner cell mass
and trophectoderm cells.
The IMO groups' blastocysts had brighter appearance, more prominent inner cell mass
and less irregularities compared with blastocysts produced in SOFaaBSA alone (Figure 1).
Cell allocation of ovine blastocysts derived from oocytes matured in different maturation media IVM media Blastocysts * Total cell ICM (1) n M[+ or -]SEM M[+ or -]SEM TCM-FCS 19 54.6[+ or -]4.6 12.6[+ or -]1.0 TCM-BSA 14 53.1[+ or -]4.5 12.7[+ or -]1.1 SOF-FCS 20 57.8[+ or -]3.9 12.5[+ or -]0.9 SOF-BSA 13 57.1[+ or -]4.5 13.1[+ or -]1.0 IVM media TE (2) ICM/TE M[+ or -]SEM %[+ or -]SEM TCM-FCS 41.9[+ or -]3.7 23.6[+ or -]0.7 TCM-BSA 40.4[+ or -]3.4 23.8[+ or -]0.9 SOF-FCS 45.3[+ or -]3.2 22.0[+ or -]1.1 SOF-BSA 44.0[+ or -]3.8 23.2[+ or -]1.2 * 6-day old blastocysts (1): Inner cell mass
; (2): Trophectoderm cells Table 3.
The inner cell mass
has undergone another fate decision and has differentiated into three layers: endoderm, mesoderm, and ectoderm.
Embryonic Stem cells (ES) cells from the inner cell mass
of normal human blastocysts were cultured and maintained normally for many passages in 1998.
These cells are derived from the inner cell mass
of 3.5-day-old blastocysts [1,2].
When removed from the blastocyst and cultured in a glass dish in the laboratory, the inner cell mass
gives rise to ESCs.
Once a fertilized egg has gone through several rounds of division, stem cells form a bulge, called the inner cell mass
, inside the early embryo.
After about five days, a blastocyst forms: Cells move to form a thin layer around the edge and a group of small cells, called the inner cell mass
, assembles along one side.
Blastocyst--A sphere made up of an outer layer of cells (the trophectoderm), a fluid-filled cavity (the blastocoel), and a cluster of cells on the interior (the inner cell mass