We performed a hemagglutination inhibition assay
by using APMV-1, APMV-2, APMV-3, APVA, and APVC antisera against isolates confirmed.
Note that letrozole is the reference chemical for the Tox21 aromatase inhibition assay
, which is consistent with its high rank.
The CPE inhibition assay
allowed us to observe a significant reduction on virus titers between untreated infected cultures ([10.
Other highlights of the study, which were presented by Adrian Wildfire, Project Director for Infectious Diseases and the Human Challenge Unit at the recent World Vaccine Congress in Washington DC, included the successful use of the microneutralisation test (MNT) in place of the haemagglutination inhibition assay
(HAI) to estimate serosusceptibility prior to challenge.
50]-CPE inhibition assay
using C6/36 cell line, dilution of virus showing 100 [TCID.
As illustrated in Figure 2, CaME at 40 ug/ml showed 50% inhibition of plaque of HSV-1 and provided 100% inhibition against HSV-1 at 200-500 ug/ml as indicated by the plaque inhibition assay
Buccal epithelial cell (BEC) adhesion inhibition assay
of toothpaste supernatant against C.
NA-Star[R] Influenza NA Inhibitor Resistance Detection Kit (Applied Biosystems, Massachusetts, USA) was employed for the NA inhibition assay
according to the manufacturer's protocol.
HA and HI tests are simple, sensitive, relatively inexpensive and easy to perform, has its limitation in requirement of continuous source of reactive RBC's and need to monitor specificity of low titred reaction with haemagglutination inhibition assay
The candidates, later, were tested for functional activity using an in vitro cell migration inhibition assay
and an in vivo tumor xenograft model in combination with carboplatin.
The BioCryst discovered compounds indicate sub-nanomolar potency on the isolated enzyme and single digit nanomolar potency in suppressing kallikrein activity in an ex vivo activated human plasma kallikrein inhibition assay
Hyaluronidase inhibition assay
was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid.