Samples from the ocular conjunctiva for microbiological examination were collected with a sterile swab in the inferior conjunctival fornix
of both eyes, avoiding touching the eyelashes or eyelids, and were placed test tubes with Stuart transport medium, which were sent to the Microbiology Laboratory of the Universidade de Brasilia (UnB).
Aseptic surgical preparation from the lower eyelid to upper lip was done and a small nick incision was made in the inferior conjunctival fornix
and directly below the upper fornix of the lip (Fig.
Cigarette ashes were noted in his lashes and inferior conjunctival fornix
at the initial examination in the emergency service.
In addition to the visual acuity testing, the ophthalmologists examined the inferior conjunctival fornix
to assess follicles and to sample when positive.