curvatus) characterised through molecular characterization was found to be negative for catalase test, indole test
(green layer is formed at the top of test tube), nitrate reduction test, citrate test (no blue colour in test tube), methyl red Voges-Proskeur test (no red colour appears in test tube), casein hydrolysis test (not able to produce protease enzyme) and for starch agar test (not able to produce amylase enzyme) (Table 1).
Then indole test
was performed to confirm the Coliforms.
This included V chromogenic agar, spot indole test
and associated labour costs.
5-1 mm in (1-2 mm in dia) dia) Catalase Test Negative Negative Positive Endospore Negative Negative Negative staining Motility Test Non motile Non motile Motile Indole Test
Negative Negative Positive Nitrate Test Negative Negative Positive [H.
coli colonies were injected into triple sugar iron and urea agar slants and subjected to the indole test
The spot indole test
was also positive and confirmatory of the identification.
Selected colonies were sub-cultured for purity onto Levine EMB and Columbia 5% sheep blood, and isolates underwent biochemical testing, including the indole test
, which with colonial morphology is a presumptive test for Escherichia coli (N=28), plating on differential media (Rainbow Agar) specific for Shiga toxin producing Escherichia coli (N=17), and latex agglutination tests specific for O157 and H7 antigens (N=17) to confirm Escherichia coli O157:H7.
The spot indole test
, completed in less than one minute, is a rapid and convenient method for the culture confirmation of H.
The biochemical tests performed were sugar fermentation tests, catalase tests, indole test
, methyl red (MR) test, Vogue's Proskauer(VP) test, lactose fermentation, urease production and citrate utilization (38).
Biochemical characterization and identification of the isolated strain of bacteria (Pasturella multocida) showed a positive oxidase, catalase and indole test
which confirmed that the death of mice was due to Pasturella multocida.
The biochemical identification tests showed--spot indole test
negative; fermentation of glucose, maltose positive; and no fermentation of lactose, sucrose and xylose was seen.
They were also positive for Voges Proskauer and negative for indole test