curvatus) characterised through molecular characterization was found to be negative for catalase test, indole test
(green layer is formed at the top of test tube), nitrate reduction test, citrate test (no blue colour in test tube), methyl red Voges-Proskeur test (no red colour appears in test tube), casein hydrolysis test (not able to produce protease enzyme) and for starch agar test (not able to produce amylase enzyme) (Table 1).
Salmonella colonies were identified using standard biochemical tests including fermentation of glucose negative urease reaction lysine decarboxylase negative indole test
H2S production and fermentation of dulcitol.
For further confirmation biochemical tests were performed such as indole test
, catalase test, triple sugar iron test and MRVP test.
This included BA/MC, 1/4 CLED agar, spot indole test
, 1/4 MacConkey agar, 1/4 Citrate agar and associated labour costs.
coli pentosaceus Gram Staining Gram positive Gram positive Gram negative Cell morphology Small rod shape Cocci shape Rod shape Colony White, small, white, smooth, Small circular circular circular (0.5-1mm in dia) Characteristic (0.5-1 mm in (1-2 mm in dia) dia) Catalase Test Negative Negative Positive Endospore Negative Negative Negative staining Motility Test Non motile Non motile Motile Indole Test
Negative Negative Positive Nitrate Test Negative Negative Positive [H.sub.2]S Gas Negative Negative Positive Production Table 2: Carbohydrate fermentation profile of the isolate DM15.
The spot indole test
, completed in less than one minute, is a rapid and convenient method for the culture confirmation of H.
A series of conventional biochemical tests such as oxidative fermentative, oxidase, nitrate reduction, arginine decarboxylase and indole test
was performed for confirmation of isolated colonies of Pseudomonas aeruginosa.7 Identified colonies were streaked onto Muller Hinton Agar plates containing 8ug/mL of Levofloxacin to select FQn resistant isolates as per CLSI instructions.8 Resistant colonies were directly transferred into PCR tubes.
Biochemical test results of endophytic phytase-producer bacterial isolates from Zea mays L No Biochemistry Test Test Result 1 TSIA (Triple Sugar - Iron Agar) Test 2 H2S Test - 3 M2otility Test - 4 Catalase Test + 5 Indole Test
- 6 Methyl Red Test - 7 Voges Proskauer - Test 8 Citrate Test + 9 Carbohydrate - Lactose Fermentation + Mannitol Glucose + Genus Burkholderia
Preliminary identification of strains obtained in pure culture was based on Gram staining and the typology process was performed by assessing the samples with catalase, coagulase, sucrose use (glucose or saccharose), indole test
, methyl red test, Voges-Proskauver test, and using the citrate as carbon source, urease activity regarding biochemical and physical properties.
Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test
(IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured.
Biochemical characterization and identification of the isolated strain of bacteria (Pasturella multocida) showed a positive oxidase, catalase and indole test
which confirmed that the death of mice was due to Pasturella multocida.
The biochemical identification tests showed--spot indole test
negative; fermentation of glucose, maltose positive; and no fermentation of lactose, sucrose and xylose was seen.