indirect immunofluorescence


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Related to indirect immunofluorescence: Flow cytometry, ELISA, FITC, QPCR

indirect immunofluorescence

fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies).
See also: fluorescent antibody technique.

in·di·rect im·mu·no·fluo·res·cence

(in'di-rekt' im'yū-nō-flōr-es'ĕns)
Fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies).
See also: fluorescent antibody technique
References in periodicals archive ?
Absorption with [alpha]-enolase before indirect immunofluorescence did not eliminate the pANCA staining pattern, which is consistent with the idea that [alpha]-enolase is not the target antigen of pANCA.
The search for ANA was conducted by indirect immunofluorescence, using API-Plus slide preparers and HEp-2 cells as substrate (Euroimmun, Lubecca, Germany), starting with an initial dilution of 1:80, and adding conjugated fluoresceinated human anti-immunoglobulin G.
Screening for autoantibodies to SSA/Ro by indirect immunofluorescence using HEp-2000 cells.
section]) Antibodies against ABV in serum sample, detected by indirect immunofluorescence assay.
In Table 2, the clinical information is summarized as a function of the indirect immunofluorescence pattern observed on the HEp-2000 cells.
All observers were experienced with reading the results indirect immunofluorescence microscopy.
IgM antibodies against the viral capsid of EBV were determined by indirect immunofluorescence (EBV IgM IFA Test-GULL).
Follow-up investigation on day 16 of illness showed an increased IgG titer (80) against chikungunya virus (by indirect immunofluorescence assay; reference value <1:20) (6) but no IgM titer.
Samples are screened with indirect immunofluorescence (IIF), and only when this test is positive are further assays for extractable nuclear antigens (ENAs) performed.
Indirect immunofluorescence assays (Focus Diagnostics, Cypress, CA, USA) performed on the paired serum samples revealed an increased A.
Anti-CCP antibodies were tested by ELISA (Immunoscan RA; EuroDiagnostica); RF was measured by laser nephelometry for the IgM isotype and by ELISA for the IgA and IgG isotypes; antinuclear antibodies were assayed by indirect immunofluorescence on HEp-2 cells.
After 6 days of propagation, ZIKV-infected cells were detected by indirect immunofluorescence assay using specific hyperimmune mouse ascitic fluids provided by the Institut Pasteur (Dakar, Senegal) (9).