Absorption with [alpha]-enolase before indirect immunofluorescence
did not eliminate the pANCA staining pattern, which is consistent with the idea that [alpha]-enolase is not the target antigen of pANCA.
The search for ANA was conducted by indirect immunofluorescence
, using API-Plus slide preparers and HEp-2 cells as substrate (Euroimmun, Lubecca, Germany), starting with an initial dilution of 1:80, and adding conjugated fluoresceinated human anti-immunoglobulin G.
Screening for autoantibodies to SSA/Ro by indirect immunofluorescence
using HEp-2000 cells.
section]) Antibodies against ABV in serum sample, detected by indirect immunofluorescence
In Table 2, the clinical information is summarized as a function of the indirect immunofluorescence
pattern observed on the HEp-2000 cells.
All observers were experienced with reading the results indirect immunofluorescence
IgM antibodies against the viral capsid of EBV were determined by indirect immunofluorescence
(EBV IgM IFA Test-GULL).
Follow-up investigation on day 16 of illness showed an increased IgG titer (80) against chikungunya virus (by indirect immunofluorescence
assay; reference value <1:20) (6) but no IgM titer.
Samples are screened with indirect immunofluorescence
(IIF), and only when this test is positive are further assays for extractable nuclear antigens (ENAs) performed.
assays (Focus Diagnostics, Cypress, CA, USA) performed on the paired serum samples revealed an increased A.
Anti-CCP antibodies were tested by ELISA (Immunoscan RA; EuroDiagnostica); RF was measured by laser nephelometry for the IgM isotype and by ELISA for the IgA and IgG isotypes; antinuclear antibodies were assayed by indirect immunofluorescence
on HEp-2 cells.
After 6 days of propagation, ZIKV-infected cells were detected by indirect immunofluorescence
assay using specific hyperimmune mouse ascitic fluids provided by the Institut Pasteur (Dakar, Senegal) (9).