indirect immunofluorescence


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Related to indirect immunofluorescence: Flow cytometry, ELISA, FITC, QPCR

indirect immunofluorescence

fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies).
See also: fluorescent antibody technique.

in·di·rect im·mu·no·fluo·res·cence

(in'di-rekt' im'yū-nō-flōr-es'ĕns)
Fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies).
See also: fluorescent antibody technique
References in periodicals archive ?
Comparison of indirect immunofluorescence and enzyme immunoassay methods for the determination of antinuclear antibodies.
In this study, the percentages of ASAs were similar in cervicovaginal secretions (62.2%) and serum (64.4%) of infertile women using two indirect agglutination and indirect immunofluorescence (IIF) tests.
Complement receptor gC1qR expression and distribution on bovine sperm plasma membrane were determined by indirect immunofluorescence, using the treatment group (50 [micro]g/ml heparin for 30 minutes, see above) with its corresponding control.
Expression of SUM-1 in the intact embryo precedes overt myogenic differentiation of the secondary mesenchyme cells as determined by RNAse protection analyses using riboprobes to SUM-1 and myosin heavy chain (MHC) and labeling of cells by indirect immunofluorescence (4, 5).
We sent his blood samples and the crust of the eschar to the Chiba Prefectural Institute of Public Health (Chiba, Japan) for indirect immunofluorescence and PCR assays (Appendix, https: //wwwnc.cdc.gov/EID/article/25/6/18-1985-App1.
ANCAs are detected by indirect immunofluorescence in approximately 20% of patients with SLE (5).
C, Ultrastructure of the viral particles consistent with the Zika viruses (indirect immunofluorescence [IIF] with maternal immunoglobulin C antibodies [A]; IIF with omission of maternal immunoglobulin C antibodies [B]; electron microscopy [C]).
According to the recent recommendations of the American College of Rheumatology, ANA Task Force, the indirect immunofluorescence (IIF) assay on human epidermoid laryngeal carcinoma cells (HEp-2 cells) remains the gold standard for ANA testing [3].
Work-up for a suspected autoimmune blistering disease includes a physical exam, histology, direct and indirect immunofluorescence, and serologic tests.
Indirect immunofluorescence did not demonstrate circulating anti-basement membrane autoantibodies in the serum.