fluorescent antibody technique

(redirected from indirect fluorescent antibody test)

fluor·es·cent an·ti·bod·y tech·nique

a technique for antigen using a fluorescent antibody, usually performed by one of two methods: direct, immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe or other), the resulting antigen-antibody complex located by fluorescence microscopy; or indirect, unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex is labeled with fluorescein-conjugated antiimmunoglobulin antibody with the resulting triple complex located by fluorescence microscopy.

fluor·es·cent an·ti·bod·y tech·nique

(flōr-es'ĕnt an'ti-bod-ē tek-nēk')
A procedure to test for antigen with a fluorescent antibody by one of two methods: direct, in which immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe, or other), the resulting antigen-antibody complex being located by fluorescence microscopy; or indirect, in which unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex may be labeled with fluorescein-conjugated anti-immunoglobulin antibody, the resulting triple complex then being located by fluorescence microscopy.

fluorescent antibody technique (FAT) or immunofluorescence

a technique used to show up the presence of a particular ANTIGEN, in which an antibody is labelled with a fluorescent dye (fluorochrome) that combines with the antigen and is subsequently located by its FLUORESCENCE.
References in periodicals archive ?
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigsPesq.
Serodiagnosis of cutaneous leishmaniasis in Jordan using indirect fluorescent antibody test and the enzyme-linked immunosorbent assay.
6) Smooth muscle IgG antibodies were tested by indirect fluorescent antibody test using antigenic substrates of rodent rat liver-kidney-stomach sections and sera diluted at 1:40.
Antibodies to rabies virus were detected in specimens of the girl's serum and cerebrospinal fluid (CSF) by indirect fluorescent antibody test (IFA).
Several different serological techniques have been proposed: complement-fixation test [4], fluorescent antibody [18,26], capillary tube agglutination test [28], rapid card agglutination test [2], indirect fluorescent antibody test [10,17] and enzyme linked immunosorbent assay (ELISA) [11,25,30,32,33].
Samples were evaluated through the indirect fluorescent antibody test (IFAT).
A number of serological tests have been used for the diagnosis of borreliosis; these include the indirect fluorescent antibody test (IFA), enzyme-linked immunosorbent assay (ELISA), and western immunoblot analysis.
PCR-positive samples and further randomly chosen serum samples (n = 47 for each community) were checked by an indirect fluorescent antibody test (IFAT) (IgG IFA kit; Fuller Laboratories, Fullerton, CA, USA) for reactivity to B.
canis antibodies may be performed by Indirect Fluorescent Antibody Test (IFAT) or Dot-ELISA (CADMAN et al.
fowleri infection requires a culture or an indirect fluorescent antibody test, which is performed at a reference laboratory (8).
Serum was tested by using an indirect fluorescent antibody test with human lung cancer cells (CRL-5803; American Type Culture Collection) for detection of antibodies against SVA (B.

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