indirect Coombs test

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in·di·rect Coombs test

(kūmz), Avoid the incorrect forms Coomb and Coomb's.
a test routinely performed in cross-matching blood or in the investigation of transfusion reaction: test for patient's serum is incubated with a suspension of donor erythrocytes; if specific antibodies are present, they become attached to the antigen in the donor cells; after a washing with saline, Coombs antihuman globulin is added; agglutination at this point indicates that antibodies present in the original test serum had indeed become attached to donor erythrocytes.

Coombs in·di·rect test

(kūmz in'dĭr-ekt' test)
Laboratory test using serum that contains an antibody that may be used for erythrocyte typing; positive (i.e., abnormal) finding in isoimmunization from previous transfusions of incorrect cross-matching.
See: Coombs test
Synonym(s): indirect Coombs test.


Robin R.A., English veterinarian and immunologist, 1921–.
Coombs serum - serum from a rabbit or other animal previously immunized with purified human globulin to prepare antibodies directed against IgG and complement. Synonym(s): antihuman globulin
Coombs test - a test for antibodies, the so-called antihuman globulin test, using either the direct or indirect Coombs tests. Synonym(s): antiglobulin test
direct Coombs test - a test for detecting sensitized erythrocytes in erythroblastosis fetalis and in cases of acquired immune hemolytic anemia.
Gell and Coombs reaction - see under Gell
indirect Coombs test - a test routinely performed in cross-matching blood or in the investigation of transfusion reaction.
References in periodicals archive ?
These antibodies can cross the placenta and may lead to a positive indirect Coombs test in the mother.
Coombs test, indirect Coombs test in the mother, and anti-E antibody levels were positive and the diagnosis of hyperbilirubinemia caused by minor blood group incompatibility due to anti-E was confirmed.
Indirect Coombs test was negative about 1 month after surgery.
The patient's Kell antigen was positive, his mother's was negative, and the maternal serum indirect Coombs test was positive; therefore, the diagnosis of Kell isoimmunization was confirmed.
Other test results (liver and renal function, serum folate and vitamin B12 levels, lactic dehydrogenase levels, C-reactive protein, serum protein electrophoresis, direct and indirect Coombs tests, and antinuclear antibody tests) were within normal limits, as were viral serologic test results (HIV, hepatitis B virus, hepatitis C virus, parvovirus B19).
Direct and indirect Coombs tests were found to be negative, indirect bilirubin value: 3.