indirect Coombs test

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in·di·rect Coombs test

(kūmz), Avoid the incorrect forms Coomb and Coomb's.
a test routinely performed in cross-matching blood or in the investigation of transfusion reaction: test for patient's serum is incubated with a suspension of donor erythrocytes; if specific antibodies are present, they become attached to the antigen in the donor cells; after a washing with saline, Coombs antihuman globulin is added; agglutination at this point indicates that antibodies present in the original test serum had indeed become attached to donor erythrocytes.
Farlex Partner Medical Dictionary © Farlex 2012

Coombs in·di·rect test

(kūmz in'dĭr-ekt' test)
Laboratory test using serum that contains an antibody that may be used for erythrocyte typing; positive (i.e., abnormal) finding in isoimmunization from previous transfusions of incorrect cross-matching.
See: Coombs test
Synonym(s): indirect Coombs test.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012


Robin R.A., English veterinarian and immunologist, 1921–.
Coombs serum - serum from a rabbit or other animal previously immunized with purified human globulin to prepare antibodies directed against IgG and complement. Synonym(s): antihuman globulin
Coombs test - a test for antibodies, the so-called antihuman globulin test, using either the direct or indirect Coombs tests. Synonym(s): antiglobulin test
direct Coombs test - a test for detecting sensitized erythrocytes in erythroblastosis fetalis and in cases of acquired immune hemolytic anemia.
Gell and Coombs reaction - see under Gell
indirect Coombs test - a test routinely performed in cross-matching blood or in the investigation of transfusion reaction.
Medical Eponyms © Farlex 2012
References in periodicals archive ?
These antibodies can cross the placenta and may lead to a positive indirect Coombs test in the mother.
Coombs test, indirect Coombs test in the mother, and anti-E antibody levels were positive and the diagnosis of hyperbilirubinemia caused by minor blood group incompatibility due to anti-E was confirmed.
Indirect Coombs test was negative about 1 month after surgery.
The presence of severe anaemia with jaundice and hepatosplenomegaly along with positive direct and indirect Coombs test led to the diagnosis of autoimmune hemolytic anaemia in this case which prompted us to use prednisolone in this case.
The combination of anemia, reticulocytopenia, hydrops fetalis, and a positive indirect Coombs test suggested Kell isoimmunization.
The direct Coombs test is positive in autoimmune haemolytic anaemia, and the indirect Coombs test may or may not be positive.
In addition, direct and indirect Coombs tests were positive.
Other test results (liver and renal function, serum folate and vitamin B12 levels, lactic dehydrogenase levels, C-reactive protein, serum protein electrophoresis, direct and indirect Coombs tests, and antinuclear antibody tests) were within normal limits, as were viral serologic test results (HIV, hepatitis B virus, hepatitis C virus, parvovirus B19).
Direct and indirect Coombs tests were found to be negative, indirect bilirubin value: 3.5 mg/dL (normal: <2 mg/dL), LDH: 800 IU/L (normal: <600 IU/L).