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Even though there were small indel mutations in the remaining three regions, no rearrangements were found.
The analysis pipe line next picks up base substitutions, short insertions and deletions (indels), copy number alterations, and gene fusions, translocations, or rearrangements.
The genotype and allele frequencies of the identified indel and SNPs in exons of MITF from four duck populations categorized into two groups based on plumage color (white and black or mixed) are shown in Table 3.
The fourth step in the pipeline uses mapping of the sequencing reads to the reference sequence to identify SNPs and indels. We perform SNP and indel determination by using SAMtools and VarScan2, which also calculate SNP frequency in the sequence data (10,11).
As for FSHb, an indel was found to be significantly associated with testis long circumference (TLC) (P = 0.050) and testis weight (TW) (P = 0.001) of 40-day-old Landrace (LD) piglets, with the BB genotype showing the largest testis measurement traits.
For indel variants, detection sensitivity and specificity were more than 99% for small indels (<5 bp) and variants within nonhomopolymer regions (<7 of the same nucleotide in a row).
3n and 3n+1 and 3n+2 in yellow circles and orange and green ovals, respectively, represent the different types of InDels caused by a specific sgRNA.
To improve SNP and indel calling, indel realignment was conducted with Genome Analysis Toolkit (GATK v3.3) .
Polymorphisms at the ACE gene, such as the insertion/ deletion (indel) variant of 287 bp in intron 16 (rs1799752), have been shown to be associated with ACE activity variations and with phenotypes of strength/power or endurance performance (8, 9).
Alu's are involved in all known classes of regulatory elements, from new exon formation and alternative splicing to gene silencing, from INDEL formation to the regulation of the lncRNAs which organize chromatin loops into functional areas.
Located within Renarte's corporate office premises, it offers clients a view of the company's product catalogue across various brands such as Paderno, Indel, Dudson, Royal Bone China and Guy Degrenne among others.
The study focuses on length mutations, intron-containing genes, grouping of genes in different identity classes based on pairwise comparison of individual genes, and InDel analysis of divergent genes.
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