in vitro transcription


Also found in: Acronyms.

in vitro transcription

a CELL-free system that contains all the necessary ENZYMES and other components for the TRANSCRIPTION of DNA sequences.
References in periodicals archive ?
For live imaging of Egr3 expression in mouse oocytes, full-length and truncated Egr3 cRNAs were produced by in vitro transcription using mMessage mMachine T7 transcription kit (Ambion, Austin, TX, USA).
Based on labeling technique, the market is segmented into PCR, nick translation, random primer, in vitro transcription, reverse transcription, and end labeling.
As a first line of screening, in vitro transcription assays are very useful due to their speed, convenience, and cost effectiveness.
The single-stranded sense and antisense DNA oligonucleotides were annealed in distilled water and 100 ng was used in 30 [micro]L of in vitro transcription reaction.
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