in situ hybridization

(redirected from in situ nucleic acid hybridization)
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in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.

in situ

[L.] in its normal place; confined to the site of origin.

in situ hybridization
see in situ hybridization of nucleic acid.
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