hybridization

(redirected from in situ hybridization of nucleic acid)
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hybridization

 [hi″brid-ĭ-za´shun]
1. the production of hybrids.
fluorescent in situ hybridization (FISH) a genetic mapping technique using fluorescent tags for analysis of chromosomal aberrations and genetic abnormalities. Called also chromosome painting.
molecular hybridization in molecular biology, formation of a partially or wholly complementary nucleic acid duplex by association of single strands, usually between DNA and RNA strands or previously unassociated DNA strands, but also between RNA strands; used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.

hy·brid·i·za·tion

(hī'brid-i-zā'shŭn),
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
3. The specific reassociation of complementary strands of polynucleic acids, for example, the formation of a DNA-RNA hybrid.
4. The process or act of forming a macromolecular hybrid in which the subunits are obtained from different sources.
Synonym(s): crossbreeding

hybridization

Molecular biology The formation of a complex of complementary nucleotides; hybridization allows determination of the relatedness or sequence 'homology' between 2 strands of nucleic acids, and precise ID of short–up to 20 kb segments of DNA–Southern blot or RNA–Northern blot

hy·brid·i·za·tion

(hī'brid-ī-zā'shŭn)
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
3. The specific association of complementary strands of polynucleic acids, e.g., the formation of a DNA-RNA hybrid.
Synonym(s): hybridisation.

hybridization

See MOLECULAR HYBRIDIZATION.

hy·brid·i·za·tion

(hī'brid-ī-zā'shŭn)
1. The process of breeding a hybrid.
2. Crossing over between related but nonallelic genes.
Synonym(s): hybridisation.
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