in situ hybridization


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Related to in situ hybridization: Fluorescent in situ hybridization

in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.
References in periodicals archive ?
- Fluorescence In Situ Hybridization (FISH) (Technique) Global Competitor Market Share Positioning for 2019 & 2025
Determination of Her-2/Neu status in breast carcinoma: comparative analysis of immunohistochemistry and fluorescent in situ hybridization. Modern Pathology 2000;13(1):3745.
In situ hybridization method, using genomic DNA of two species as a probe, is an effective way to approach the individual chromosome identification of nonsomatic hybrids [16].
Chivukula on several projects, including the HER2 dual in situ hybridization (DISH) project.
Defining the efficiency of fluorescence in situ hybridization on uncultured amniocytes on a retrospective cohort of 27407 diagnosis.
Deterction of streptococcus suis by in situ hybridization, indirect immunofluorescent, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissues sections from pigs.
Genomic in situ hybridization (GISH) and RFLP analysis for the identification of alien chromosomes in the backcross progeny of potato (+) tomato fusion hybrids.
Parental origin of the extra chromosome in the cat eye syndrome: Evidence from heteromorphism and in situ hybridization analysis.
With in situ hybridization the proble is applied directly to a tissue section.
(4, 5) In addition to reducing the threshold for positive and equivocal HER2 status on immunohistochemistry (IHC), the HER2:chromosome enumeration probe 17 (CEP17) ratio used to define a positive in situ hybridization (ISH) result decreased from [greater than or equal to] 2.2 to [greater than or equal to] 2 irrespective of HER2 copy number.
Reveal Biosciences is a San Diego-based company that's developing the next generation of quantitative histopathology, combining the latest artificial intelligence techniques with high-quality histology, immunohistochemistry and in situ hybridization to generate enhanced data from tissue samples.
(1) These guidelines included specific recommendations for HER2 immunohistochemistry (IHC) as well as in situ hybridization (ISH) in formalin-fixed, paraffin-embedded (FFPE) tissues.