in situ hybridization


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Related to in situ hybridization: Fluorescent in situ hybridization

in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.

in situ

[L.] in its normal place; confined to the site of origin.

in situ hybridization
see in situ hybridization of nucleic acid.
References in periodicals archive ?
Chivukula on several projects, including the HER2 dual in situ hybridization (DISH) project.
Deterction of streptococcus suis by in situ hybridization, indirect immunofluorescent, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissues sections from pigs.
9) More recently, molecular techniques such as Southern blot hybridization, polymerase chain reaction, and in situ hybridization demonstrated direct evidence for an association between EBV and Hodgkin lymphoma.
Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.
Here, HPV testing by in situ hybridization can be very helpful.
Development of automated brightfield double in situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (CEN17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence in situ hybridization (FISH).
Analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for EpsteinBarr viral genomes by in situ hybridization.
Comparative genomic hybridization (CGH) is a molecular cytogenic technique that enables simultaneous enumeration of every chromosome, in contrast to fluorescent in situ hybridization (FISH), which at present can karyotype only a maximum of nine chromosomes, Leeanda Wilton, Ph.
Breakthrough advancements in Fluorescence In Situ Hybridization take Molecular Cytogenetics market to an entirely new level
Contract award notice: Supply, Installation, Commissioning, Training, Technical support and guarantee of an immunomarking and in situ hybridization automaton
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