in situ hybridization


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Related to in situ hybridization: Fluorescent in situ hybridization

in situ hybridization

a technique developed in 1969 for annealing nucleic acid probes to cellular DNA for detection by autoradiography. Under proper laboratory conditions, the binding process occurs spontaneously. In situ hybridization constitutes a key step in DNA fingerprinting.

in situ hybridization

A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells; ISH is used to map gene sequences to chromosomal sites and detect gene expression. See cDNA, Chromosome, CISH–Chromogenic in situ hybridization, DNA sequence, Eukaryote, FISH–Fluorescent in situ hybridization, GISH–Genomic in situ hybridization, Gene expression, Gene mapping, Hybridization, Immunoblotting, Interphase, Probe. Cf Southern blot.

in si·tu hy·brid·i·za·tion

(in sī'tū hī'brid-ī-zā'shŭn)
A technique for annealing nucleic acid probes to cellular DNA for detection by autoradiography; a key step in DNA fingerprinting.

in situ hybridization

HYBRIDIZATION of particular NUCLEIC ACID sequences, for example mRNA, in cells or tissues.

in situ

[L.] in its normal place; confined to the site of origin.

in situ hybridization
see in situ hybridization of nucleic acid.
References in periodicals archive ?
Comparison of fluorescence in situ hybridization (FISH) and dual-ISH (DISH) in the determination of HER2 status in breast cancer.
Fluorescence in situ hybridization is the only technique that can be applied to superficial melanocytic tumors or melanocytic tumors in which the malignant component may consist of a relatively small proportion of the overall cell population, as well as large bulky tumors.
9) More recently, molecular techniques such as Southern blot hybridization, polymerase chain reaction, and in situ hybridization demonstrated direct evidence for an association between EBV and Hodgkin lymphoma.
Dual-color silver-enhanced in situ hybridization for assessing HER2 gene amplification in breast cancer.
Comparison of multitarget fluorescence in situ hybridization in urine with other noninvasive tests for detecting bladder cancer.
Spindle cell malignant lymphoepithelial lesion of the parotid gland: Clinical, light microscopic, ultrastructural, and in situ hybridization findings in one case.
Breakthrough technological advancements in Fluorescence In Situ Hybridization as well as array-based technology have taken the Cytogenetics market to an entirely new level in the areas of research and molecular diagnostics.
Within 36-48 hours, in situ hybridization gave the same results as tissue culturing for all patients.
The second edition of In Situ Hybridization Protocols has a new editor and is substantially different in its stated aims and intended audience.
Thus far, attempts to detect measles virus in otosclerotic bone by tissue culturing or localizing the virus by in situ hybridization have failed.
For this new edition contributors focus on advances in non-isotropic in situ hybridization and amplification techniques for tissues and cells, including PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection and quantification methods.
We used albino frogs for in situ hybridization studies of Xslug mRNA expression and pigmented frogs for all other studies.
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