impression cytology

cytology, impression 

A simple, non-invasive means of studying cells on the conjunctiva. It is carried out by pressing a small piece of special filter paper against the anaesthetized bulbar conjunctiva for a few seconds, after which it is removed. The operation is usually repeated two or three times over the same area. The filter paper is then fixed to a glass slide, stained and examined under a microscope. Mucin, goblet cells and many epithelial cells which stain in different colours, depending on the dyes used, can be assessed and facilitate the diagnosis of many external eye diseases, such as keratoconjunctivitis sicca and xerophthalmia. See Gram stain.
References in periodicals archive ?
Conjunctiva impression cytology is one of the most practical, fast, safe and economical method to examine the changes of conjunctiva cells.
This study was planned to examine the changes in the upper tarsal conjunctiva in new continuous gas-permeable contact lens (CWRGP) wearers using impression cytology procedure.
To the best of our knowledge, there is no reported data available on subjects wearing CWRGP using impression cytology methods.
However, samples of conjunctiva cells were collected only at baseline and after 3 months of wearing the lenses using impression cytology.
Impression cytology has been used widely to evaluate several ocular surface disorders, monitoring the effect of treatment such as contact lens wear and documenting sequential changes in the conjunctival and corneal surface over time (1, 11-13).
4% of the asymptomatic soft contact lens wearing eyes showed abnormal conjunctiva impression cytology changes within the same period (6).
Interestingly, in recent times, impression cytology has proved to be very effective in the diagnosis of ocular surface diseases, better understanding of the pathophysiology of the diseases related to ocular surface and provides biomarkers which could be used as outcome measures (7).
This study concludes that well adapted and asymptomatic continuous RGP wearers for 3 months show no adverse effects to their conjunctival epithelia as measured by impression cytology.
Conjunctival impression cytology in dry eye states.
Staging of conjonctival squamouse metaplasia by impression cytology.
Cellulose asetate impression cytology of the ocular surface-dry eye state.
The outcome of such an agreement is very promising, thus, the initial results of our collaboration will be presented at the Association for Research in Vision and Ophthalmology (ARVO) 2011 Annual Meeting in May on a poster on the evaluation of membranes material for impression cytology.