impression cytology

cytology, impression 

A simple, non-invasive means of studying cells on the conjunctiva. It is carried out by pressing a small piece of special filter paper against the anaesthetized bulbar conjunctiva for a few seconds, after which it is removed. The operation is usually repeated two or three times over the same area. The filter paper is then fixed to a glass slide, stained and examined under a microscope. Mucin, goblet cells and many epithelial cells which stain in different colours, depending on the dyes used, can be assessed and facilitate the diagnosis of many external eye diseases, such as keratoconjunctivitis sicca and xerophthalmia. See Gram stain.
References in periodicals archive ?
While Frozen Section tissue architecture closely approximates permanent histology sections, enabling a degree of comfort, Touch Impression Cytology provides better, crisp cellular details and even some tissue architecture with fewer artefacts.
Conjunctival impression cytology and the morphology of epithelial cells were observed in both groups of subjects.
The tears and conjunctival cells of the patients were collected to perform: TFT, impression cytology, and MUC5AC levels.
The most widely used diagnostic tests for tear film examination are tear break-up time (TBUT) test, corneal and conjunctival staining with fluorescein, rose bengal and lissamine green dye, Schirmer test, tear film osmolarity test and impression cytology.
Impression cytology has also been utilised to look at matrix metalloproteinase 9 (MMP-9) which is upregulated in those with DED and may be another possible biomarker for the condition.
Ocular Surface Disease Index (OSDI) questionnaire, break-up time (BUT) test, corneal fluorescein staining, corneal and conjunctival rose Bengal staining, Schirmer test, and conjunctiva impression cytology were performed sequentially on days 0 and 91.
Conjunctiva impression cytology is one of the most practical, fast, safe and economical method to examine the changes of conjunctiva cells.
Staging of conjonctival squamouse metaplasia by impression cytology. Ophthalmol 1985; 92: 728-33.
The subjects were submitted to the following tests, exactly in the order cited, during the first visit (T0) and at follow-up visits (T1 and T3): best spectacle-corrected visual acuity (BSCVA) converted for LogMar scale, tear film osmolarity by electrical conductivity [16], biomicroscopy [17], Schirmer 1 test without anesthesia [18], tear film breakup time (TBUT) [18], fluorescein staining [19], completing patient's symptoms questionnaire and ocular surface disease index (OSDI) [20], lissamine green staining [18], and impression cytology (IC) and staining by periodic acid Schiffhematoxylin (PAS-H).
Impression cytology refers to the application of cellulose acetate filter paper to the ocular surface to remove the superficial layers of the ocular surface epithelium.