In our case, the patient had no family history of any neoplasms and the fumarate hydratase immunostain
was negative, making this diagnosis unlikely.
The most common stain used was Melan-A/MART-1 (melanoma antigen recognized by T cells), the most specific of the IHC markers available and the one "largely responsible for the increased incidence in overall immunostain
use in our study," the researchers wrote.
Tissue specimens for histological examination can provide the opportunity to immunostain
the tissue, further increasing differential diagnostic capabilities for suspected metastatic lesions.
Immunohistochemistry staining used neurofilaments protein immunostain
(showing bundles of axons), synaptophysin immunostain
(showing cytoplasm of ganglion cells and also axons), and neuronal nuclear immunostain
(showing the ganglion cells' cytoplasm and nuclei) (Figure 6).
Caption: FIGURE 1: The neoplastic lymphoid follicles ((a), H&E stain, 400x) are of B-cell lineage ((b), CD20 immunostain
, 400x), are admixed with a subset of CD3 positive T-cells ((c), CD3 immunostain
, 400x), coexpress CD10 ((d), CD10 immunostain
, 400x) and bcl2 positivity ((e), bcl2 immunostain
, 400x), and have proliferation rates of approximately 20-30% ((f), MIB-1 immunostain
Upon immunohistochemical staining, however, the "immunostain
panel does not support epithelioid sarcoma, melanoma, angiosarcoma, malignant peripheral nerve sheath tumor, renal cell carcinoma, or leiomyosarcoma," said the researchers.
It is important to be aware of epithelioid angiosarcoma, as its histologic appearance and positive cytokeratin immunostain
may mimic a poorly differentiated carcinoma.
In order to evaluate the degree of proliferating blood vessels we used the proliferating-endothelium marker CD105 and we compared the results with those obtained with CD31 immunostain
. Histopathological examination of the sections was carried out under an optical microscope.
 whereby immunohistochemical stains were performed on 45 out of the 351 cases showed that only vimentin was consistently immunoreactive in all the 45 CCSK cases, while CD99 was consistently negative and TLE1 immunostain
was never performed.
Out of 37 malignant cases 24/37 (65%) lesions showed Positivity for this immunomarker while 13/60 (35%) showed negativity for the immunostain
. Galectin-3 helped in the preoperative diagnosis of 100% (23/23) follicular adenomas, 75% (21/28) papillary carcinomas and 33.3% (3/9) follicular carcinomas.
The overall recurrence rate was 8%, and there were no recurrences with use of the MART-1 (melanoma antigen recognized by T-cells 1 staining) immunostain
, said Dr.