A controlled and blinded study of immunoreactant
deposition at the dermalepidermal junction of patients with primary fibrositis syndrome.
load was assessed by the LRA testing.
An experienced observer will be familiar with background fluorescence and the positive control area for each immunoreactant
This is mainly to overcome the problem of direct conjugation of HRP to immunoreactant
with bifunctional coupling reagents.
The capillary wall distribution of immunoreactants
is rarely seen in IgA nephropathy.
Direct immunoflourescence studies for immunoreactants
in colloid bodies and dermoepidermal junction will further aid in establishing a diagnosis of interface dermatitis.
Because the interaction of antibody and antigen is not associated with a quantifiable physical or chemical change, the binding event is measured by an auxiliary reaction in which one of the immunoreactants
is labeled with a substance that can easily be detected by spectrophotometry (29).
Other histologic findings that may be seen include extravasated erythrocytes granulocytic debris (leukocytoclasis) granulomatous or lymphocytic inflammation and deposition of immunoreactants
in the vessel wall.
Fibrillary glomerulonephritis may be confused with membranous glomerulopathy when a granular pattern of fluorescence predominates, because the positive immunoreactants
and the distribution of the staining pattern are similar to what is typically seen in membranous nephropathy.
Direct immunofluorescence (DIF) microscopy of a sample of perilesional skin can show tissue-bound immunoreactants
Taking a sample from the blister itself can cause degeneration of the immunoreactants
, leading to a false-negative impression.
When healthy, any foreign immunoreactants
(antigens) that gain entry are promptly identified, engulfed, and recycled by our ample supply of dendritic (phagocytic) surveillance cells.