(b) Cells were treated with BSA-AGEs (50 [micro]g/ml) for indicated times, anti-MDM2 antibody was used for immunoprecipitation
, and anti-Rb antibody was used for Western blot analysis, which showed the binding between MDM2 and Rb.
(a-c) Western blotting after immunoprecipitation
of Syntaxin-1 (a), Syntaxin-2 (b), and Snap25 (c) on purified synaptosomes lysates extracted from adult mice cortex.
Capture of AECA Target Antigens with Immunoprecipitation
. To identify AECA target antigens, the updated procedure shown in Figure 2 was used.
Abbreviations NO: Nitric oxide LPS: Lipopolysaccharide Pam3CSK4: Synthetic bacterial lipopeptide Pam3Cys-Ser-(Lys)4 Poly(I:C): Polyinosinic:polycytidylic acid L-NAME: L-NG-nitroarginine methyl ester MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide HPLC: High-performance liquid chromatography RT-PCR: Reverse-transcriptase polymerase chain reaction iNOS: Inducible NO synthase COX-2: Cyclooxygenase-2 TNF-[alpha]: Tumor necrosis factor-alpha IP: Immunoprecipitation
Src: Nonreceptor tyrosine kinase Syk: Spleen tyrosine kinase TAK1: Transforming growth factor beta-kinase 1.
Then, a reverse immunoprecipitation
experiment was performed using an anti-FLAG antibody.
The following primary antibodies were used: NF-[kappa]B p65 (chromatin immunoprecipitation
(ChIP) grade, #ab7970) was obtained from Abeam (Cambridge, MA, USA).
Chromatrap has announced a web page that brings together protocols developed using its proprietary solid state chromatin immunoprecipitation
Methods: Binding capacity of mutated TP53 in MDA-MB-468 breast cancer cell line to BRCA1 and RAD51 proteins in comparison to WT TP53 in MCF7 cell line was studied by Immunoprecipitation
. In vitro studies were performed by GST-WT p53 pull-down assays in these cell lines to assess the interaction of GST-WT p53 with BRCA1 and RAD51 proteins.
EMD Millipore, the Life Science division of Merck KGaA, has introduced the Magna Nuclear RNA-binding Protein Immunoprecipitation
The levels of plasma A[beta]s and A[beta]APs were measured by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) followed by immunoprecipitation
Later on Liu (2002) used monoclonal antibody PAb204 for immunoprecipitation
of HeLa nuclear extract it precipitated p68.
Called SIF-seq, for site-specific integration fluorescence-activated cell sorting followed by sequencing, this new technique developed by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) complements existing genomic tools, such as ChIP-seq (chromatin immunoprecipitation
followed by sequencing), and offers some additional benefits.