The subject of the contract is the supply, delivery, installation by the Contractor of the following equipment: immunoassay analyser, genetic analyser and equipment for immunomagnetic
separation of blood cells (bone marrow cells) in 3 (three) lots.
It enriches disease specific tumor cells using magnetic particles in an antibody mixture, an approach called immunomagnetic
cell enrichment, then isolates and purifies messenger RNA (mRNA) for analysis using reverse transcription polymerase chain reaction (RT-PCR).
In this approach, which is based on the AdnaTest[TM] platform for CTC detection, CTCs are isolated through immunomagnetic
enrichment on the basis of 2 epithelial epitopes, EpCAM (an isolation strategy that is common to the CellSearch approach) andMUC1 (mucin 1, cell surface associated) and are further analyzed downstream by multiplex RT-PCR, targeting EpCAM, MUC1, cancer antigen (CA)-125, and ERCC1 transcripts.
According to a new market report published by Transparency Market Research "Circulating Circulating Tumor Cells (CTCs) Prognostic Technologies Market[Technologies: Tumor Cell Enrichment (Filtration, Centrifugation and Immunological & Immunomagnetic
Methods) & Tumor Cell Detection (Molecular Methods and Optical Methods) and Applications: Prostate Cancer, Breast Cancer, Colorectal Cancer and Others] - Global Industry Analysis, Size, Share, Growth, Trends and Forecast, 2014 - 2020," the global circulating tumor cells (CTCs) prognostic technologies market was valued at USD 0.
The Captivate' range of immunomagnetic
separation products has also been extended to increase to eight the number of products available for the isolation of Ship Toxin-Producing Escherichia coli (STEC) strains.
Scientists have developed a rapid method for detecting and identifying Salmonella serovars, using immunomagnetic
separation (IMS) technology, Fourier-transform infrared (FTIR) spectroscopy and multivariate analysis.
Oocysts in wastewater and stream water samples were isolated from the contaminating debris by immunomagnetic
separation (IMS), and DNA was extracted (online Technical Appendix).
Escherichia coli O157 samples were enriched in tryptic soy broth (TSB), exposed to an immunomagnetic
separation step and then cultured on cefixime potassium tellurite sorbitol MacConkey (CT SMAC) and Rainbow agar containing novobiocin and tellurite (NT Rainbow) as previously described (Cooley et al.
RC confirmation: Immunomagnetic
separation followed by streaking on CT-SMAC.
All samples were tested at the Public Health Laboratory in Toronto, Ontario by routine enteric diagnostic methods, including testing of food and water samples by immunomagnetic
separation (IMS) of STEC O157 and selective culture methods based on FDA and Health Canada methods.
The researchers used immunomagnetic
separation and biotinyl-tyramide signal amplification in the assay to increase specificity and sensitivity.
The FISH analysis used by the team detected more circulating abnormal cells -- by orders of magnitude -- than existing methods that rely on immunomagnetic
beads to attach to an antibody found on the surface of circulating cells that originate in an organ's epithelium.