The methods of obtaining BMSCs mainly include density gradient centrifugation, flow cytometry, immunomagnetic
beads sorting and all bone marrow adherent culture, among them, whole bone marrow adherent culture is the simplest way to obtain the cells based on the adhesion characteristics of BMSCs (Neuhuber et al., 2008; Lin et al., 2018; Liu et al., 2018).
The MNC fraction was then enriched for CD34 cells using a CD34 + immunomagnetic
isolation kit (Miltenyi Biotec, USA) according to the manufacturers' instructions.
Detection of Clonorchis sinensis circulating antigen in sera from Chinese patients by immunomagnetic
bead ELISA based on IgY.
Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic
separation and direct plating methods.
Drawing on advances in CRISPR/Cas9 biology, we describe here a method of enriching for minor alleles by use of a deactivated Cas9 (dCas9) protein and immunomagnetic
In this way, numerous methods are found in the literature, which practically include three main steps: concentration (by sedimentation, centrifugation or filtration); purification (solutions of sodium acetate, acetic acid, zinc sulfate, ethanol or immunomagnetic
separation), and detection of (oo)cysts (direct immunofluorescence reaction, FISH--Fluorescence in situ hydridization).
The efficiency of such immunomagnetic
carriers is considered to be nearly identical to that of biotinstreptavidin systems, yet they are simple and cost-effective.
Fluorescent Antibodies and Immunomagnetic
Beads Used for FACS Analysis and Sorting.
T cell capture from blood for PCR analysis using microfluidic systems," Lab on a Chip , vol.
According to the manufacturer's instructions, peripheral blood mononuclear cells (PBMCs) were separated by Ficoll-Hypaque density gradient centrifugation (Amersham Biosciences, Piscataway, NJ, USA), and untouched [CD4.sup.+] T cells were isolated from PBMCs by immunomagnetic
beads (Miltenyi Biotec, Auburn, CA, USA).