immunoelectron microscopy


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immunoelectron microscopy

[im′yənō′ilek′tron]
electron microscopy of specimens labeled with antibodies that have been conjugated with gold. The gold makes the antibody labels electron-dense.

microscopy

examination with a microscope.

confocal microscopy
a technique for obtaining high resolution images and 3-D reconstructions of biological specimens; a laser light beam is expanded to make optimal use of the optics in the objective lens and is turned into a scanning beam via an x-y deflection mechanism and is focused to a small spot by the objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and after conversion into a static beam by the x-y scanner device is focused onto a photodetector (photomultiplier) via a dichroic mirror (beam splitter) to create the final image. Called also laser scanning microscopy; confocal scanning laser microscopy.
immunoelectron microscopy
the mixing of antibody with an antigen such as a virus on a specimen grid so as to increase the probability of visualizing a virus and to identify (type) the kind of virus present in the specimen, or antibody may be conjugated with gold and used to visualize and determine the location of specific antigenic determinants on a specimen.
References in periodicals archive ?
Atypical amyloidosis: diagnostic challenges and the role of immunoelectron microscopy in diagnosis.
The use of post-embedding immunoelectron microscopy in the diagnosis of glomerular diseases: comparison of immunoelectron microscopic and immunofluorescence studies.
The material covers basic methods of antigen selection and preparation, antibody production, and reagent manufacture, as well as a number of advanced protocols such as techniques for making new monoclonal cell lines, producing monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.
Fixation may interfere with antibody binding and thus preclude infectivity tests and successful application of any immunoelectron microscopy.