Maternal and neonatal samples were kept in the refrigerator and analysed, on a daily basis, for prolactin by a fluorescence immunoassay that uses a sandwich immunodetection
method [AFIAS prolactin kit (SMFP-8), Biotechnology, Boditech, Korea].
: After electrophoresis, the proteins were transferred to a nitrocellulose membrane (Bio-Rad 162-0112) using a transfer buffer (glycine 0.192 M; Tris 0.025 M; and 20 % methanol v/v); the transfer was carried out 1:30 hours using a Criterion-Blotter (Bio-Rad 170-4071) at 120 V immersed in ice.
and molecular determination of visceral and cutaneous Leishmania infection using patients' urine.
The effect of type of incubation and two different antigen retrieval methods on the immunodetection
of Mannheimia hemolytica antigens in archived caprine lung tissues.
However, at present, PRRSV infection is confirmed using techniques such as serology (antibody testing), molecular biology (PCR, in situ hybridization, etc.), and immunodetection
assays (immunohistochemistry, immunofluorescence, etc.).
Voluntary ex ante transparency notice: Delivery of certified antibodies (ce ivd) for clinical diagnosis, Immunodetection
antibodies (ruo) from becton dickinson for units of the silesian medical university in katowice (poland-katowice: Chemical reagents)
was not performed for the aforementioned bivalve ECM components because specific antibodies were lacking for them, and the bivalve larvae were small and difficult to cultivate.
, the proteins were separated by 12.5% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) transfer membranes (Amersham Biosciences, Piscataway, NJ, USA).
In our previous research, several tailored specific antibodies were designed for the immunodetection
of ancient silks [28, 29], leathers , and proteinaceous binders .
Thus, the reduced expression of PEPCK may be the consequence of cell death after extensive glycogen accumulation [7,9] rather than a direct downregulating effect exerted by NaW on the expression of gluconeogenic enzymes, as reduced immunodetection
is only observed in proximal tubules that show severe glycogenic alterations.
[39 ] analyzed 174 ANA-positive samples by immunodiffusion, LIA, and an automatized fluorescent immunodetection
methodology, showing no difference between them.
Because the p38 kinases are responsive to stresses such as cytokine stimulation and curcumin inhibits p38 MAPK phosphorylation in RAW 264.7 murine macrophages , immunodetection
of phospho-p38 was performed under similar conditions.