Briefly, the samples were lysed using lysis buffer, centrifuged at 14,000 g for 30 min at 4[degrees]C and the supernatant was used for immunodetection
with anti TH (1:500 dilution), anti TuJl(l [micro]g/ml) and anti [beta]-catenin (0.
was performed by using 2 PrP-specific monoclonal antibodies, Sha31 (1 [micro]g/mL) (20), and 12B2 (4 [micro]g/mL) (21), which recognize amino acid sequences YEDRYYRE (145-152), and WGQGG (89-93), respectively.
for the TNF-[alpha]-like protein was very weak.
Degradation of cardiac troponin I: implication for reliable immunodetection
of DNA repair endonuclease ERCC1-XPF in human tissue.
Horseradish peroxidase conjugated goat anti-rabbit or goat anti-mouse IgGs and the SuperSignal west Pico Chemiluminescent substrate (Thermo Scientific) were used for immunodetection
Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection
and expression in laser microdissected tissues.
Previously, we reported that the immunodetection
of cytoplasmic membrane-bound TM in UC, squamous cell carcinoma and adenocarcinoma formalin fixed paraffin embedded tissue microarrays was independent of grade and stage.
Thyroid peroxidise immunodetection
as a tool to assist diagnosis of thyroid nodules on fine needle aspiration biopsy.
Two of the differentially expressed proteins were selected for immunodetection
in undepleted plasma using specific antibodies to integrin alpha-11 and isoform-1 of multimerin-1 and the differential serum levels were confirmed by Western blotting analysis [Figure 3].
Thyroid peroxidase immunodetection
as a tool to assist diagnosis of thyroid nodules on fine-needle aspiration biopsy.
Dementia in Parkinson's disease: biochemical evidence for cortical involvement using the immunodetection
of abnormal Tau proteins.