The authors measured relative [beta]-cell mass volume using Image-Pro Plus software (Media Cybermetric, Silber Springs, MD), the frequency of [beta]-cell apoptosis by terminal deoxynucleotydyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and replication index using Ki-67 immunocytochemical staining
for specific markers such as keratin 3, vimentin and MUC5AC, was used to identify cells in the graft.
Different markers typical of stem cells were tested and specific stages of germ cells by immunocytochemical staining
showed the expression of transcription factor (self renewal function) such as OCT4 and SOX2 in the MSCs culture.
A study by Beraki et al (20) was aimed at establishing a protocol for immunocytochemical staining
and chromogenic ISH of Giemsa- and Diff-Quick (Fisher Scientific, Pittsburgh, Pennsylvania)-prestained cytologic smears.
In our case, immunocytochemical staining
with S-100 and Melan-A protein were positive with the tumour cells, allowing a definitive diagnosis of metastatic melanoma.
The supernatant was discarded and the sediment, after tipping the bottom of the tube several times, was smeared on four slides, two of them were ordinary glass slides for conventional cytological examination using Papanicolaou-stain, and the other two slides were positive charged slides for immunocytochemical staining
with CAL and CEA using Flex Monoclonal Mouse Anti-Human Calretinin (ready to use) of Clone: DAK-Calret 1, Isotope: IgG1, kappa, Code No.
5 shows representative immunocytochemical staining
for 8-OHdG in HepG2 cells with or without tacrine treatment.
After incubation in presence of appropriate secondary antibodies, immunocytochemical staining
was performed using the streptavidin/peroxidase method (SP kit) according to manufacturer's instructions (Zhongshan Biotechnological Ltd.
Additionally, cell proliferation was also examined using cell cycle assay by flow cytometry and immunocytochemical staining
for proliferating cell nuclear antigen (PCNA).
Antibody production in rabbits and immunocytochemical staining
utilized standard methods (7).