immunoblot


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Related to immunoblot: immunoprecipitation

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', im'yū-nō-blot'ing),
Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
See also: Western blot analysis.
Farlex Partner Medical Dictionary © Farlex 2012

immunoblot

(ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', -ing)
Process by which antigens can be separated by electrophoresis and blotted to nitrocellulose sheets, where they bind and are subsequently identified by staining with labeled antibodies.
See also: Western blot analysis
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Out of 100 clinically diagnosed typhoid fever cases 16(16%) were blood culture positive for Salmonella typhi, 58(58%) were widal test positive and 74(74%) were immunoblot positive (Table-1).
immunoblot despite high C6 reactivity, and a negative PCR on DNA obtained from the skin biopsy argue against co-infection.
Immunoblot analysis showed that the interaction of caspase-1 and caspase-4 was prevented by NleF but not NleF ([DELTA]4aa).
In PBC patients, Western immunoblot detects AMA significantly more often than indirect immunofluorescence on HEp-2 cells (85% versus 72%, P=0.02) or rodent tissue sections (71%, P=0.01)5.
coli LPS-induced NF-[kappa]B, the level of the cytoplasm of the I[kappa]B-[alpha] protein was measured through an immunoblot analysis.
Furthermore, to evaluate whether this data was consistent with a G2 block, we performed an immunoblot with an antibody specific for phospho-[Ser.sup.10] Histone 3 (pH3).
The immunoblot should not be run if the EIA or IFA tests are negative.
In the current study, the immunoblot analysis with individual patient's serum demonstrated diverse IgE reactivity with seven proteins of 15, 23, 28, 39, 45, 66, and 85 kDa as major IgE binding components.
Hass, "Comparison of Mycoplasma bovis strains based on SDS-PAGE and immunoblot protein patterns," Journal of Veterinary Medicine B, vol.
hermsii antibodies by either enzyme immunoassay (EIA) or immunoglobulin M (IgM) and immunoglobulin G (IgG) Western immunoblots. A sample collected from the newborn 3 days later had equivocal results by EIA and three bands visible on IgM immunoblot and one band visible on IgG immunoblot.