immunoblot


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Related to immunoblot: immunoprecipitation

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', im'yū-nō-blot'ing),
Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
See also: Western blot analysis.

immunoblot

/im·mu·no·blot/ (im´u-no-blot″) a technique for, or the blot resulting from, analyzing or identifying proteins via antigen-antibody specific reactions, as in Western blot technique.

immunoblot

(ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies.

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', -ing)
Process by which antigens can be separated by electrophoresis and blotted to nitrocellulose sheets, where they bind and are subsequently identified by staining with labeled antibodies.
See also: Western blot analysis

immunoblot

References in periodicals archive ?
The immunoblot should not be run if the EIA or IFA tests are negative.
In fact, Ang and others (7) highlight potential performance differences among Borrelia tier-one tests, tier-two immunoblots, and especially the combination of pairing them together.
Western immunoblot confirmed that nonphosphorylated and phosphorylated 4E-BP1 were expressed in reactive nodes, FL, and DLBCL (Figure 2, N).
The protein identified was further confirmed by immunoblot analysis in which a recombinant C.
By means of special tests like gel electrophoresis, immunoblot and immunoblot inhibition, two proteins called cross-reactive allergens were identified.
The samples were dried by vacuum evaporation, solubilized by boiling for 5 min in Laemmli sample buffer, and then subjected to electrophoresis and immunoblot analysis with MAB 1H12 as described previously.
One of the most popular type-specific gG-based serology commercial kits is the HerpeSelect ELISA test for HSV-1 and HSV-2 made by Focus, followed by the HerpeSelect Immunoblot for HSV-1 and HSV-2, also made by Focus; the biokitHSV-2, a 6-minute test designed for in-office use made by biokit USA Inc.
Using immunoblot analysis and antibodies raised against RB38, RB47 and RB60 from C.
Sera were examined by the enzyme-linked immunosorbent assay (ELISA) and western immunoblot assay using whole cell preparations as antigen to detect equine IgG antibodies to the spirochete Borrelia burgdorferi.
However, immunoblot analysis, using antibodies raised against vertebrate Gs revealed an immunoreactive band of the predicted size ([sim]49kD) in Y-organ and control tissue (blue crab thoracic ganglion and mouse brain).
monocytogenes have been incorporated into a microcolony immunoblot technique that diagnoses and enumerates the pathogenic L.