immunoblot


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Related to immunoblot: immunoprecipitation

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', im'yū-nō-blot'ing),
Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
See also: Western blot analysis.

immunoblot

/im·mu·no·blot/ (im´u-no-blot″) a technique for, or the blot resulting from, analyzing or identifying proteins via antigen-antibody specific reactions, as in Western blot technique.

immunoblot

(ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies.

im·mu·no·blot

, immunoblotting (im'yū-nō-blot', -ing)
Process by which antigens can be separated by electrophoresis and blotted to nitrocellulose sheets, where they bind and are subsequently identified by staining with labeled antibodies.
See also: Western blot analysis

immunoblot

References in periodicals archive ?
In PBC patients, Western immunoblot detects AMA significantly more often than indirect immunofluorescence on HEp-2 cells (85% versus 72%, P=0.
coli LPS-induced NF-[kappa]B, the level of the cytoplasm of the I[kappa]B-[alpha] protein was measured through an immunoblot analysis.
The first tier uses a highly sensitive EIA or IFA that, if the result is positive or equivocal, is followed by a highly specific Western immunoblot as the second-tier test
Furthermore, to evaluate whether this data was consistent with a G2 block, we performed an immunoblot with an antibody specific for phospho-[Ser.
We first examined LC3 conversion and p62 protein degradation, two widely used markers of autophagic flux [25], in hearts by immunoblot analysis.
12) The patient reported consuming raw freshwater fish in his diet, and the immunoblot assay of CSF identified reactive antibodies to a specific 24-kDa band diagnostic of gnathostomiasis.
The immunoblot should not be run without first performing an EIA or IFA.
Com proteins were quantified; immunoblot analysis determined the abundance.
Those found to be positive by screen and IgG positive by immunoblot should be reported as positive, and those positive by screen and IgM positive by immunoblot should be reported as positive; however, that positive IgM result is only clinically significant in very early disease.
The key message is that it must always be a two-step process: Use an enzyme immunoassay or immunofluorescence assay as a screening test and, if it's positive or equivocal, send the specimen for a standard IgM/IgG Western immunoblot.