immunoassay


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immunoassay

 [im″u-no-as´a]
any of several methods for the quantitative determination of chemical substances, such as hormones, drugs, and specific proteins, that utilize the highly specific binding between an antigen or hapten and homologous antibodies, including radioimmunoassay, enzyme immunoassay, and fluoroimmunoassay.

im·mu·no·as·say

(im'yū-nō-as'ā, im-yū'nō),
Detection and assay of substances by serologic (immunologic) methods; in most applications the substance in question serves as antigen, both in antibody production and in measurement of antibody by the test substance.
See also: enzyme-linked immunosorbent assay, radioimmunoassay, radioimmunoelectrophoresis, immunologic pregnancy test.

immunoassay

/im·mu·no·as·say/ (-as´a) quantitative determination of antigenic substances (e.g., hormones, drugs, vitamins) by serological means, as by immunofluorescent techniques, radioimmunoassay, etc.

immunoassay

(ĭm′yə-nō-ăs′ā, ĭ-myo͞o′-)
n.
A laboratory technique that identifies and quantifies a protein, such as a hormone or an enzyme, based on its ability to act as an antigen or antibody in a chemical reaction.

immunoassay

[im′yənō·as′ā]
Etymology: L, immunis + Fr, essayer, to try
a commonly employed assay in which a solid-phase target antigen is designed to bind with an antibody in vitro to reveal its presence. When a conjugated antihuman immunoglobulin is added, it binds the antibody of interest. The conjugate, usually an enzyme, then reacts with its substrate to generate color or fluorescence that is proportional to antibody concentrations. See also enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), sandwich technique.

immunoassay

Lab medicine Any assay that measures an antigen-antibody response, the sensitivity of which varies according to the method; an immunologic test that quantifies a particular antigen using a cognate antibody. See ELISA, RIA.

im·mu·no·as·say

(im'yū-nō-as'ā)
Detection and assay of substances by serologic (immunologic) methods.
See also: radioimmunoassay, radioimmunoelectrophoresis, immunologic pregnancy test
Synonym(s): immunochemical assay.

immunoassay

A method of testing for, and measuring the concentration of, various antibodies. Various methods, including RADIOIMMUNOASSAY, enzyme linked immunosorbent assay (ELISA) and immunoelectrophoresis may be used.

immunoassay

an assay of a substance that is based on its reaction with a specific ANTIBODY. See, for example, ELISA.

immunoassay

detection and assay of serum antigenic agents by antibody titre

im·mu·no·as·say

(im'yū-nō-as'ā)
Detection and assay of substances by serologic (immunologic) methods; in most applications substance in question serves as antigen, both in antibody production and in measurement of antibody by the test substance.

immunoassay (im´ūnōas´ā),

n a competitive-binding assay in which the binding protein is an antibody.

immunoassay

the quantitative determination of either antibody or antigen, e.g. hormones, drugs, vitamins, and specific proteins, by means of antigen-antibody interaction, as by agglutination, precipitation, ELISA, radioimmunoassay, etc.
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5) Healthy study participants who consumed 15 mg to 30 mg of biotin daily were shown to have biotin concentrations of over 30 ng/mL; these levels could interfere with biotin-based immunoassays.
The other, published April 20 in Analytical Chemistry, demonstrates the effect in immunoassays.
In addition, although almost all of the participants using immunoassays with a cutoff of 300 ng/mL detected the opiate hydromorphone at 1000 ng/mL, the many laboratories that used fluorescent immunoassay (FIA) and microparticle immunoassay (kinetic interaction of microparticles in solution) did not (UDS-A 2003) (Table 2).
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