immunoaffinity purification

immunoaffinity purification

(ĭ-mū″nō-ă-fĭn′ĭ-tē, ĭ-mū″)
Exposure to monoclonal antibodies in order to isolate specific analytes. Immunoaffinity purification is used during the preparation of blood products to reduce their likelihood of contamination by specific disease-causing microorganisms.
References in periodicals archive ?
Characterizing antibody cross-reactivity for immunoaffinity purification of analytes prior to multiplexed liquid chromatography-tandem mass spectrometry.
Characterization of SF-Derived Exosomes Isolated by the Immunoaffinity Purification Method.
In particular, the tag appended to the N-terminus of the fusion protein is necessary for the immunoaffinity purification with M1 monoclonal antibody, while M2 is position-insensitive.
The material covers basic methods of antigen selection and preparation, antibody production, and reagent manufacture, as well as a number of advanced protocols such as techniques for making new monoclonal cell lines, producing monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.
Heavy-isotope-labeled ([sup.13]C-[sup.15]N-Lys/Arg) recombinant tau was spiked into CSF samples before immunoaffinity purification and subsequent digestion and analysis.
The 2 methods were relatively poorly correlated, which may be due to the improved specificity of the immunoaffinity purification approach (see Fig.
The immunoaffinity purification of the sample therefore yielded a much cleaner preparation than the SPE procedures, which result in multiple peaks even when analyzed by tandem MS (10,11).
Fractions containing proteins smaller than 20 kDa were collected and subjected to immunoaffinity purification as described above.
In the case of the reports in this issue of Clinical Chemistry (1, 2), the samples were prepared for analysis by a single-step immunoaffinity purification that can be carried out in large numbers using a standard 96-well microtiter plate format.
Immunoaffinity purification of creatine kinase-MB from human, dog, and rabbit heart with use of a monoclonal antibody specific for CK-MB.
Immunoaffinity purification and gas chromatography-mass spectrometric quantitation of 3-alkyladenines in urine: metabolism studies and basal excretion levels in man.