15]N-Lys/Arg) recombinant tau was spiked into CSF samples before immunoaffinity purification
and subsequent digestion and analysis.
The material covers basic methods of antigen selection and preparation, antibody production, and reagent manufacture, as well as a number of advanced protocols such as techniques for making new monoclonal cell lines, producing monoclonal antibodies, immunocapture PCR, immunoaffinity purification
, and immunoelectron microscopy in plant virus diagnosis.
PhosphoScan([R]) profiling involves immunoaffinity purification
and tandem mass spectroscopy and its effectiveness in kinase target and biomarker discovery in cancer was illustrated most recently in the journal Cell (Rikova K.
The brief communication by Laha and coworkers in the current issue of Clinical Chemistry, entitled "Characterizing Antibody Cross-reactivity for Immunoaffinity Purification
of Analytes prior to Multiplexed Liquid Chromatography-Tandem Mass Spectrometry," is an excellent example of the power of this combined approach (1).
CST) announced today that it will make PhosphoScan[R] Profiling, CST's proprietary technology based on immunoaffinity purification
and tandem mass spectrometry, and information within PhosphoSite[R], CST's knowledgebase focused on phosphorylation of the mammalian proteome, available to academic and non-profit researchers.
The 2 methods were relatively poorly correlated, which may be due to the improved specificity of the immunoaffinity purification
approach (see Fig.
The PhosphoScan([R]) strategy involves immunoaffinity purification
and tandem mass spectroscopy, and it was first described in the journal Nature Biotechnology, Rush J.
The immunoaffinity purification
of the sample therefore yielded a much cleaner preparation than the SPE procedures, which result in multiple peaks even when analyzed by tandem MS (10,11).
It involves a patent-pending method for immunoaffinity purification
of phosphopeptides coupled to mass spectroscopic sequence and phosphorylation site determination.
Fractions containing proteins smaller than 20 kDa were collected and subjected to immunoaffinity purification
as described above.
The PhosphoScan(R) technology involves immunoaffinity purification
and mass spectroscopy and was described in the journal Nature Biotechnology (Rush J.
The PhosphoScan(TM) technology involves immunoaffinity purification
and mass spectroscopy and was described in the journal, Nature Biotechnology (Rush J.