immunoadsorbent


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immunoadsorbent

 [im″u-no-ad-sor´bent]
a preparation of antigen attached to a solid support or antigen in an insoluble form, which adsorbs homologous antibodies from a mixture of immunoglobulins.
References in periodicals archive ?
Another important issue was to demonstrate that Ro and La ribonucleoproteins were citrullinated, and present results demonstrated citrullinated proteins eluted from the immunoadsorbent of Sepharose-4B coupled to anti-citrulline antibodies; therefore, the complex of citrullinated proteins was in the range of 60-48 kDa, and this means that PAD enzymes drive a wide citrullination process including other than Ro60 and La, also probably the albumin can be citrullinated, which is a frequent contaminant of cell extracts and is within the range of molecular weight.
Sharma, Modified hydroxyapatite microspheres as immunoadsorbent for plasma perfusion: preliminary study, J.
After eliminating the cross-reacting antibody using an immunoadsorbent, a strong precipitin line was observed between the egg protein and the antibody in the double immunodiffusion.
Cytokine concentrations were measured in culture supernatants by enzyme-linked immunoadsorbent assay (ELISA) after centrifugation (R&D Systems, Minneapolis, MN, USA).
anthracis DNA by polymerase chain reaction from specimens collected from an affected tissue or site; or the presence of anti-protective antigen immunoglobulin G (anti-PA IgG) by enzyme-linked immunoadsorbent assay (3).
Immunoadsorbent membranes are not inert; they may generate free radical production and trigger the activation of cytokines, clotting and complement factors, and interleukin 1.
Serum antibody responses in naturally occurring influenza A virus infection determined by enzyme-linked immunoadsorbent assay, hemagglutination inhibition and complement fixation.
In short, after in vitro iron saturation of transferrin (mixing 50 [micro]L of serum sample, 200 [micro]L of ferric citrate solution, and 1 mL of buffer) and adsorption of isotransferrins with isoelectric point values <5.7 (non-CDT-isotransferrins) on the anion-exchange microcolumns, the isotransferrins with isoelectric point >5.7 (CDT-isotransferrins) in the column effluxes were determined by means of a competitive, double-antibody immunoadsorbent assay.
NE levels were determined by Enzyme Linked Immunoadsorbent Assay (NE ELISA kits, Uscn Life Science Inc.
The simple production of IgE (analysis by in vitro methods, i.e., enzyme-linked immunoadsorbent assay) and binding of IgE to relevant proteins (sodium dodecyl sufate-polyacrylamide gel electrophoresis/ immunoblot analysis) should be considered insufficient to establish a protein as an allergen without clinical relevance.
NAG isoenzymes A and B were purified from human placentas by procedures including chromatographies on concanavalin A-Sepharose (Pharmacia, Uppsala, Sweden), an immunoadsorbent column, Mono Q Sepharose (Pharmacia), and Superose 12 column (Pharmacia) by the method of Kinoshita et al.