Another important issue was to demonstrate that Ro and La ribonucleoproteins were citrullinated, and present results demonstrated citrullinated proteins eluted from the immunoadsorbent
of Sepharose-4B coupled to anti-citrulline antibodies; therefore, the complex of citrullinated proteins was in the range of 60-48 kDa, and this means that PAD enzymes drive a wide citrullination process including other than Ro60 and La, also probably the albumin can be citrullinated, which is a frequent contaminant of cell extracts and is within the range of molecular weight.
Sharma, Modified hydroxyapatite microspheres as immunoadsorbent
for plasma perfusion: preliminary study, J.
After eliminating the cross-reacting antibody using an immunoadsorbent
, a strong precipitin line was observed between the egg protein and the antibody in the double immunodiffusion.
Cytokine concentrations were measured in culture supernatants by enzyme-linked immunoadsorbent
assay (ELISA) after centrifugation (R&D Systems, Minneapolis, MN, USA).
anthracis DNA by polymerase chain reaction from specimens collected from an affected tissue or site; or the presence of anti-protective antigen immunoglobulin G (anti-PA IgG) by enzyme-linked immunoadsorbent
membranes are not inert; they may generate free radical production and trigger the activation of cytokines, clotting and complement factors, and interleukin 1.
Serum antibody responses in naturally occurring influenza A virus infection determined by enzyme-linked immunoadsorbent
assay, hemagglutination inhibition and complement fixation.
In short, after in vitro iron saturation of transferrin (mixing 50 [micro]L of serum sample, 200 [micro]L of ferric citrate solution, and 1 mL of buffer) and adsorption of isotransferrins with isoelectric point values <5.7 (non-CDT-isotransferrins) on the anion-exchange microcolumns, the isotransferrins with isoelectric point >5.7 (CDT-isotransferrins) in the column effluxes were determined by means of a competitive, double-antibody immunoadsorbent
NE levels were determined by Enzyme Linked Immunoadsorbent
Assay (NE ELISA kits, Uscn Life Science Inc.
The simple production of IgE (analysis by in vitro methods, i.e., enzyme-linked immunoadsorbent
assay) and binding of IgE to relevant proteins (sodium dodecyl sufate-polyacrylamide gel electrophoresis/ immunoblot analysis) should be considered insufficient to establish a protein as an allergen without clinical relevance.
NAG isoenzymes A and B were purified from human placentas by procedures including chromatographies on concanavalin A-Sepharose (Pharmacia, Uppsala, Sweden), an immunoadsorbent
column, Mono Q Sepharose (Pharmacia), and Superose 12 column (Pharmacia) by the method of Kinoshita et al.