The content of tumor necrosis factor (TNF)-[alpha], C-reactive protein (CRP), soluble intercellular adhesion molecule (SICAM)-1 and interferon (IFN)-I3 were detected using enzyme-linked immunoabsorbent
Applications: Enzyme-linked Immunoabsorbent
Assay; Western Blot (Recombinant Protein); Antibody Production; Protein Array
IL-10 and IL-17A were measured by enzyme-linked immunoabsorbent
IgA from faecal homogenates was quantified using a sandwich enzyme-linked immunoabsorbent
assay (ELISA) technique with a Rat IgA ELISA Quantification Set (E110-102) from Bethyl Laboratories (Montgomery, TX, USA).
MCP-1 concentrations in 3T3-L1 adipocytes supernatants were measured by using enzyme-linked immunoabsorbent
From the two blood samples obtained at day 0 and day 57, serum samples were analyzed for IGF-1, GH, (Enzo Life Sciences, Plymouth Meeting, PA, USA), and HGF (Ray Biotech, Norcross, GA, USA) using commercially available enzyme-linked immunoabsorbent
assay (ELISA) kits.
Plasma concentrations of oxytetracycline were determined by competitive enzyme-linked immunoabsorbent
assay, and pharmacokinetic parameters were obtained.
Although both enzyme-linked immunoabsorbent
assay (ELISA) and indirect hemagglutination have been used as screening tests for zoonotic Dirofilaria infections, neither have proven sensitive or specific enough for human diagnoses, and both exhibit a wide range of antibody cross-reactivities with other nematodes.
Serum Xaa-pro dipeptidase/prolidase (PEPD) was measured using an enxyme-linked immunoabsorbent
assay (ELISA) test kit (Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit Cusabio biotech)according to the manufacturer's procedure.
Serum and urinary TNF-[alpha] levels were measured by an enzyme-linked immunoabsorbent
assay (ELISA) using commercially available standard kits (Quantikine high-sensitivity human TNF-[alpha] Research & Diagnostic Systems, Europe Ltd, Abington, UK) in the Laboratory of Kidney Disease Research of the Department of Nephrology at "Hippokratio" General Hospital.
Blood was centrifuged for ten minutes at 13000 rpm and plasma was separated for biochemical analysis of cytokines (IL- 6 and IL-18) through enzyme-linked immunoabsorbent
assay ELISA by using the diagnostic kits [MBL for interleukin-18 and Invitrogen CMC0063 for interleukin 6] following manufacturer's recommendations.
A presenca e a concentracao do CTV nas plantas foram determinadas por ELISA indireto (Indirect Enzyme-Linked Immunoabsorbent
Assay (KOENIG, 1981).