MCP-1 concentrations in 3T3-L1 adipocytes supernatants were measured by using enzyme-linked immunoabsorbent
From the two blood samples obtained at day 0 and day 57, serum samples were analyzed for IGF-1, GH, (Enzo Life Sciences, Plymouth Meeting, PA, USA), and HGF (Ray Biotech, Norcross, GA, USA) using commercially available enzyme-linked immunoabsorbent
assay (ELISA) kits.
Plasma concentrations of oxytetracycline were determined by competitive enzyme-linked immunoabsorbent
assay, and pharmacokinetic parameters were obtained.
Although both enzyme-linked immunoabsorbent
assay (ELISA) and indirect hemagglutination have been used as screening tests for zoonotic Dirofilaria infections, neither have proven sensitive or specific enough for human diagnoses, and both exhibit a wide range of antibody cross-reactivities with other nematodes.
Serum Xaa-pro dipeptidase/prolidase (PEPD) was measured using an enxyme-linked immunoabsorbent
assay (ELISA) test kit (Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit Cusabio biotech)according to the manufacturer's procedure.
Blood was centrifuged for ten minutes at 13000 rpm and plasma was separated for biochemical analysis of cytokines (IL- 6 and IL-18) through enzyme-linked immunoabsorbent
assay ELISA by using the diagnostic kits [MBL for interleukin-18 and Invitrogen CMC0063 for interleukin 6] following manufacturer's recommendations.
A presenca e a concentracao do CTV nas plantas foram determinadas por ELISA indireto (Indirect Enzyme-Linked Immunoabsorbent
Assay (KOENIG, 1981).
Urinary albumin concentration was measured by enzyme-linked immunoabsorbent
assay using an anti-rat albumin antibody and 24 h protein excretion was calculated by multiplying the urinary protein excretion by the 24 h urine volume.
beta] and IL-6 by sandwich enzyme linked immunoabsorbent
assay (ELISA): Aliquots of 100 [micro]l from collected serum samples or standard proteins of [IL-1.
assay (ELISA) was used for qualitative determination of IgG antibodies (HpIgG ELISA) to Helicobacter pylori in serum.
in Pomona, California, for screening using enzyme-linked immunoabsorbent
assays followed by verification of positive samples with mass spectral detection using liquid chromatography/mass spectrometry.
An indirect immunoabsorbent
enzyme assay (ELISA) was used to assay the sera.