Indeed, this result about the optimum pH alteration was expected because of the context of the environments of free and immobilized enzymes
as previously indicated.
Furthermore, immobilized enzymes
are easily separated from products and used in the continuous production processes in the industry.
The activity of the immobilized enzymes
reached a maximum value at about 50[degrees]C, compared to around 40 and 50[degrees]C for the native [beta]-galactosidase and galactose oxidase, respectively, reported previously [57, 58].
The effects of pH and temperature variations on the hydrolytic activity of the immobilized enzymes
There are some reports showing efforts to unveil the properties and characteristics of new hybrid materials with an organic/inorganic composition seeking future prospects for industrial applications of immobilized enzymes
requiring nonharmful or toxic agents [7, 13].
Reusability of Immobilized Enzymes
Under MW Irradiation
HPAC and affinity chromatography have also been employed in examining the binding of various solutes and drugs with the transport proteins [[alpha].sub.1]-acid glycoprotein (AGP) or human serum albumin 1HSA) (10, 17, 18), the binding of urediofibrate-like dual agonists with peroxisome proliferator-activated receptors (19), and the interactions of many compounds with immobilized enzymes
or lectins (12, 20--22).
Because enzymes are generally sensitive to heat, enhanced thermal stability is among the most important properties of immobilized enzymes
The response of the biosensors as a function of the relative concentration of the two immobilized enzymes
has been investigated and the best working conditions have been identified by measuring the linear range response and sensitivity of each prepared biosensor.
The pH is an important factor that influences the hydrolytic activity of both free and immobilized enzymes
, since it can change the performance of the support, the enzyme conformation and the enzymatic reaction.
The RA can be considered more precise immobilization yield than IY since the first is based on the enzyme activity measured in the derivatives (immobilized enzymes