yield was calculated considering the total activity of the free lipase in solution given on the immobilization process (that considers the volume of the enzymatic extract employed on the immobilization test and its activity [U m[L.
Nessler ammonia assay method was used for the determination of the urease activity of both free and immobilized
The effect of variation the substrate's temperature on the free and immobilized
[beta]-galactosidase activities were investigated, by using lactose as substrates, and the obtained data are shown in Figure 4.
The thermostability of the immobilized
enzyme was characterized by thermal gravity analysis (TGA) and the experiment was performed under the nitrogen atmosphere with a heating rate of 10[degrees]C/min over a temperature range of 20-400[degrees]C using SDT-Q600.
The stability of free and immobilized
lipase was determined by measuring the residual activity against various temperature ranging from 40-80[degrees]C.
During pH stability studies of soluble and immobilized
enzymes the activity buffer was replaced with the same buffer used during the stability studies (50 mM sodium acetate buffer (C[H.
Comparatively, whole cells produced more glutamic acid than immobilized
Moreover, temperature-optimum was broadened from 50 to 60[degrees]C for the immobilized
enzyme (Figure 3).
The effects of optimum temperature on the free and immobilized
enzyme were determined by assaying their activities at different temperatures (0 to 80[degrees]C) in 50 mM sodium phosphate (pH 7.
The physical structure and chemical composition of support can influence the micro-environment of the immobilized
enzymes, and consequently their biological properties [2, 3].
Serum was collected from 86 free-ranging muskoxen chemically immobilized
or shot in northern Canada and 5 tame muskoxen for analysis of biochemistry parameters and cortisol levels (Table 1).
Currently microbial cells are immobilized
to produce a number of products like enzymes and antibiotics.