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During the prepupal stage, pb-GAL4 was expressed strongly in the salivary glands, brain, PNS (Keilin's organs and an anterior sensory complex potentially the labial sensory organ), and labial imaginal discs (Figure 2(F)).
For the imaging of imaginal discs, Yokogawa CSU10 spinning disc confocal microscopy with a Nikon eclipse E1000 microscope and a Hamamatsu Electron Multiplier CCD Digital Camera C9100-13 was used.
Taken together, we conclude that the Ubx protein trap shows a spatial and temporal expression pattern consistent with the previously reported endogenous pattern in both the embryo and imaginal discs.
Double staining of the Hth-YFP protein trap and the endogenous protein in [hth.sup.CPTI000378] imaginal discs (as well as embryos) also shows that they have similar temporal and spatial expression patterns (Figures 4 and 5).
Cell proliferation patterns in the wing imaginal disc
We chose these particular groups of late third-instar larvae because they are often collected for sampling of larval tissues and imaginal discs. The only visible difference between the two groups is crawling activity; however, changes in endogenous [Beta]-galactosidase activity may take place in the nonactive larvae as they approach the prepupal stage.
In the late third-instar larva, X-gal staining of [Beta]-galactosidase activity was found in the anterior and posterior spiracles, larval midintestine, lymph glands, cellular epidermis and in imaginal discs of the eye and antenna [ILLUSTRATION FOR FIGURE 2 OMITTED!.
Schmidt (1930) published a description of larval development in Cerebratulus marginatus from fertilization to the formation of imaginal discs, but did not observe late development or metamorphosis.
After 22 d, all three pairs of imaginal discs are present (Fig.