Because endonuclease stops the DNA chain of degradation of apoptotic cells between nucleosomes, apoptotic cells can be detected by measuring the quantity of hypodiploid
DNA inside the cells.
nuclei representing apoptosis induction in uninfected and E.
Data showed that EVO significantly reduced the viability of respective cancer cells associated with the occurrence of apoptotic characteristics such as DNA ladders, hypodiploid
cells, and activation of caspase-3.
Compared with control group cells, the MFH cells exhibited a notable hypodiploid
peak ahead of the G1 peak.
After 48 h treatment with fucoxanthin (20 [micro]M), the level of apoptotic cells characterized by DNA fragmentation showed an increased percentage of hypodiploid
cells, morphological changes, and cleavages of caspase-3 and PARP (poly(ADP-ribose) polymerase) escalated to 30% .
and hyperdiploid cells and some polyploid cells did emerge in the cultures with increased passaging , the incidence of such cells was still very small in our study (below 2%).
In the literature, hypodiploid
chromosome numbers were found in 3-10% of adults and 1-7% of childhood ALL.
The resulting percentages of apoptotic cells were determined as the percentages of hypodiploid
cells (sub G0/G1 peaks).
and hyperdiploid karyotypes were defined as having <45 and >46 chromosomes, respectively.
As shown in Figure 3B, the specific inactivation of DR4/DR5 receptors caused a 50% reduction in the amount of hypodiploid
cells, as observed after Pcy treatment (Figures 2A and 3A).
Genetic aberrations in hypodiploid
breast cancer: frequent loss of chromosome 4 and amplification of cyclin D1 oncogene.
In the hybrid hypodiploid
metaphase spreads, two intact sets of H.