Twenty-five nanograms of DNA was used to generate amplicons spanning the V3 and V4 hypervariable regions
of the bacterial 16S rRNA gene using the following primers: F-V3f 5'-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG ACY CCT ACG GGR GGC AGC AG-3' and V4r 5'-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3' (Klindworth et al., 2013).
(12) examined two hypervariable regions
of the mtDNA in a larger cohort constituting individuals well distributed in different geographical regions of Anatolian peninsula.
Mostly researchers target hypervariable regions
for studies on the taxonomy and antigenic properties of adenoviruses.
The complementarity-determining region sequences in IgY antivenom hypervariable regions
. Data Brief, v.
The 16S rRNA gene sequence has a characteristic organization, with 9 hypervariable regions
separating 10 conserved regions (Figure 1) [9,26].
Positive specimens were molecularly typed, based on sequencing of hexon hypervariable regions
1-6 and inoculated into A549 cells for virus isolation.
Humanized antibodies are produced by grafting murine hypervariable regions
on amino acid domains into human antibodies.
The RDP Pyro aligner does not align the hypervariable regions
while the Mothur aligner does using the Silva alignment as reference sequences.
To test this, we considered a large genetic data set of two hypervariable regions
(V5 and V6), from previously generated barcoded 16S ribosomal (rDNA) amplicons from microbialite communities distributed in different geographical regions of Mexico.
The typing kit targets the most hypervariable regions
of the MHC class I and class II genes.
Antigen-antibody reactions involve complementary binding between an antigen molecule surface epitope and hypervariable regions
of antibody molecules .