Effect of butylated hydroxytoluene
on bull spermatozoa frozen in egg yolk- citrate extender.
The IC50 values were calculated and compared with standard antioxidant (butylated hydroxytoluene
Survival and fertility of boar spermatozoa after freezethawing in extender supplemented with butylated hydroxytoluene
This experiment was designed to evaluate the effect of butylated hydroxytoluene
supplementation in extender on motility, plasmalemma and viability of Sahiwal bull spermatozoa.
One of the antioxidants supplemented to extender is butylated hydroxytoluene
(BHT), and its beneficial effects have been reported in many studies (Hammerstedt et al., 1976; Pursel, 1979; Watson and Anderson, 1983; Killian et al., 1989; Bamba and Cran, 1992; Donoghue and Donoghue, 1997; Roca et al., 2004; Khalifa et al., 2008; Shoae and Zamiri, 2008; Ijaz et al., 2009).
(BHT), a well-known anti-oxidant, was used as a positive control.
Extra vitamin E (20 [micro]mol/L) and butylated hydroxytoluene
(10 [micro]mol/L) were added to the aliquots as antioxidants to prevent the in vitro oxidation of LDL-cholesterol in plasma.
As for BHT (butylated hydroxytoluene
), some studies say that it causes cancer while others say that it prevents the disease.
The ovaries and young fruits of approximately 0.5 g were separately ground with 5 mL 80% (v/v) methanol extraction medium containing 0.1% butylated hydroxytoluene
and 0.06% polyvinylpyrrolidone (PVP) per gram fresh material in an ice-cooled mortar.
Chemicals: Xanthine oxidase, allopurinol, xanthine, hydroxylamine, N-(1-naphthyl)-ethylenediamine dihydrochloride, sulfanilic acid, ethylenediamine tetra acetate (EDTA), butylated hydroxytoluene
(BHT), gallic acid, 2, 4, 6-tripyridyl-s-triazine (TPTZ), 1,1-diphenyl-2- picrylhydrazyl (DPPH), acetylthiocholine iodide, 5,5- dithiobis [2-nitro benzoic acid (DTNB) were purchased from Merck (Germany) and sigma aldrich.
Piittmann, "Analysis of the antioxidant butylated hydroxytoluene
(BHT) in water by means of solid phase extraction combined with GC/MS," Water Research, vol.
Ferrozine [3-(2-pyridyl)-5, 6-bis (4-phenylsulfonicacid)-1,2,4-triazine)], Iron(II) chloride (Fecl2), butylated hydroxytoluene
(BHT), were purchased from Sigma (Germany).