This project concerns the development of fundamental knowledge of catalysis by MBL fold hydroxylases
and application of the knowledge to identify template inhibitors, which can be modified to enable selective inhibition of specific family members.
Structural insight into the aromatic amino acid hydroxylases
and their disease-related mutant forms.
The metabolism of PB is inhibited, whereas that of PM is variably affected - hydroxylases
are up-regulated and demethylases are down-regulated.
As the data presented are the first indications of anthraquinones affecting a steroid hydroxylase
system, comparative evaluations of the impact of anthraquinones on steroid hydroxylases
generally also will be most informative.
The hydroxylation of proline and lysine residues by the collagen hydroxylases
is coupled with a stoichiometric decarboxylation of 2-oxoglutarate.
Inside the renal tubular cells, DBP is degraded and 25 (OH) D is released for metabolism by 1 [alpha] or 24 hydroxylases
. There are many intracellular D binding proteins (DBP) which bind vitamin D and regulate their intracellular metabolism (8).
and the protein disulfide isomerase subunit of prolyl 4hydroxylases.
When screening for other genetic traits containing homologous genes such as CYP11B1 and CYP11B2 hydroxylases
, mixed primer amplification combined with size differentiation would help ensure that both chromosomes amplify.
The oxygen sensor enzymes, HIF-prolyl hydroxylases
(PHD1-3) prevent HIF activity under normoxic conditions.
Nickel depletes intracellular ascorbate, which leads to the inhibition of cellular hydroxylases
, manifested by the loss of hypoxia-inducible factor (HIF)-1[alpha] and -2[alpha] hydroxylation and hypoxia-like stress.
The mutant cysteine residue is one of five cysteine residues also conserved in the other human aromatic amino acid hydroxylases
, tryptophan hydroxylase
and phenylalanine hydroxylase
Control of the steroidogenic metabolic pathway is regulated partly by P450 cytochrome hydroxylases
, and the expression of many of these enzymes is controlled by the orphan nuclear receptor, steroidogenic factor-1 (SF-1).