The combination of this free radical with superoxide ([O.sub.2.sup..-]) yields urate hydroperoxide
. Both urate free radical and urate hydroperoxide
are much stronger oxidants than hydrogen peroxide and superoxide and can easily oxidize neighboring molecules increasing tissue damage and inflammation.
Polyunsaturated fatty acids (PUFA) are vulnerable to oxidation mediated by hydroperoxides
formation producing smaller volatile oxidative compounds .
The main anthropometric and biochemical serum parameters, including the levels of markers of oxidative damage to lipids (hydroperoxides
) and proteins (AOPP), ceruloplasmin, Lp-PLA2, and paraoxonase as well as TRAIL serum levels of the cohort of subjects (78% women) enrolled in the present study, are summarized in Table 1.
The oxo-degradation is an abiotic process and, thanks to the incorporation of oxygen in the polyethylene molecule, functional groups that contribute to the generation of peroxides and hydroperoxides
were assessed by colorimetric assay based on the reaction between these lipid peroxidation by-products and N,N-diethyl-paraphenylendiamine as we previously described in detail [11, 17].
Mitochondrial phospholipid hydroperoxide
glutathione peroxidase suppresses apoptosis mediated by a mitochondrial death pathway.
Immediately before and after the 1st (baseline), 5th, 10th, and 15th training sessions, oxidative stress markers (plasma lipid hydroperoxides
and plasma uric acid), plasma antioxidant capacity (6-hydroxy-2,5,7, 8-tetramethylchroman-2-carboxylic acid [TROLOX]), arterial blood gases, and acid-base balance analyses were performed.
Characteristics of hydroperoxide
are determined with their hydrocarbon component.
dissolved in olive oil was used as a standard compound for POV analysis.
Makino et al., "Formation of Ne(hexanonyl)lysine in protein exposed to lipid hydroperoxide
. A plausible marker for lipid hydroperoxide-derived protein modification," Journal of Biological Chemistry, vol.
Girotti, "Lipid hydroperoxide
generation, turnover, and effector action in biological systems," Journal of Lipid Research, vol.
Myeloperoxidase activity and lipid hydroperoxide
levels were significantly higher in operating room personnel than in the non-operating room personnel (pless than 0.001; pless than 0.001), while catalase activity and sulfhydryl group levels were significantly lower (pless than 0.009; pless than 0.003).